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Blood, 1 December 2007, Vol. 110, No. 12, pp. 3891-3899. Prepublished online as a Blood First Edition Paper on August 15, 2007; DOI 10.1182/blood-2006-10-048991. This Article Full Text (PDF) Supplemental Methods All Versions of this Article: blood-2006-10-048991v1 110/12/3891    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Oh, I.-Y. Articles by Kim, H.-S. Search for Related Content PubMed PubMed Citation Articles by Oh, I.-Y. Articles by Kim, H.-S. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted October 6, 2006 Accepted July 25, 2007 Involvement of E-selectin in recruitment of endothelial progenitor cells and angiogenesis in ischemic muscle Il-Young Oh, Chang-Hwan Yoon, Jin Hur, Ji-Hyun Kim, Tae-Youn Kim, Choon-Soo Lee, Kyung-Woo Park, In-Ho Chae, Byung-Hee Oh, Young-Bae Park, and Hyo-Soo Kim* Department of Internal Medicine, Innovative Research Institute for Cell Therapy (IRICT), Seoul National University Hospital, Seoul, Korea, Republic of * Corresponding author; email: hyosoo{at}snu.ac.kr. E-selectin plays critical roles in tethering leukocytes to endothelial cells (ECs). We studied the role of E-selectin in endothelial progenitor cell (EPC) homing and vasculogenesis. After ischemia, the expression of E-selectin on ECs peaked 6-12 h and returned to baseline at 24 h whereas the level of soluble E-selectin (sE-selectin) in serum increased over 24 h and remained high at day 7. Mouse bone marrow-derived EPCs expressed not only E-selectin but also its ligand. Homing of circulating EPCs to ischemic limb was significantly impaired in E-selectin knock-out mice, as well as wild type mice pretreated with blocking antibody against E-selectin, which was rescued by local sE-selectin injection. Mechanism for this is that sE-selectin stimulated not only ECs to express ICAM-1, but also EPCs to secrete IL-8, leading to enhanced migration and incorporation to EC's capillary formation. In therapeutic aspect, local treatment with sE-selectin enhanced efficacy of EPC transplantation for vasculogenesis and salvage of ischemic limb. Conversely, when E-selectin was knocked-down by E-selectin small interfering RNA, blood flow recovery after EPC transplantation was significantly impaired. But this impaired vasculogenesis was rescued by sE-selectin. In conclusion, these data demonstrate E-selectin is a pivotal molecule for EPCs' homing to ischemic limb and vasculogenesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Novel player in cell recruitment Stefanie Dimmeler Blood 2007 110: 3821-3822. [Full Text] [PDF] This article has been cited by other articles: M. Miljkovic-Licina, P. Hammel, S. Garrido-Urbani, P. F. Bradfield, P. Szepetowski, and B. A. Imhof Sushi repeat protein X-linked 2, a novel mediator of angiogenesis FASEB J, December 1, 2009; 23(12): 4105 - 4116. [Abstract] [Full Text] [PDF] S. Rey, K. Lee, C. J. Wang, K. Gupta, S. Chen, A. McMillan, N. Bhise, A. Levchenko, and G. L. Semenza Synergistic effect of HIF-1{alpha} gene therapy and HIF-1-activated bone marrow-derived angiogenic cells in a mouse model of limb ischemia PNAS, December 1, 2009; 106(48): 20399 - 20404. [Abstract] [Full Text] [PDF] D. P Sieveking and M. K. Ng Cell therapies for therapeutic angiogenesis: back to the bench Vascular Medicine, May 1, 2009; 14(2): 153 - 166. [Abstract] [PDF] A.O. Robb, N.L. Mills, I.B.J. Smith, A. Short, O. Tura-Ceide, G.R. Barclay, A. Blomberg, H.O.D. Critchley, D.E. Newby, and F.C. Denison Influence of menstrual cycle on circulating endothelial progenitor cells Hum. Reprod., March 1, 2009; 24(3): 619 - 625. [Abstract] [Full Text] [PDF] N. Krankel, R. G. Katare, M. Siragusa, L. S. Barcelos, P. Campagnolo, G. Mangialardi, O. Fortunato, G. Spinetti, N. Tran, K. Zacharowski, et al. Role of Kinin B2 Receptor Signaling in the Recruitment of Circulating Progenitor Cells With Neovascularization Potential Circ. Res., November 21, 2008; 103(11): 1335 - 1343. [Abstract] [Full Text] [PDF] G. Tabatabai, C. Herrmann, G. von Kurthy, M. Mittelbronn, S. Grau, B. Frank, R. Mohle, M. Weller, and W. Wick VEGF-dependent induction of CD62E on endothelial cells mediates glioma tropism of adult haematopoietic progenitor cells Brain, October 1, 2008; 131(10): 2579 - 2595. [Abstract] [Full Text] [PDF] S. Dimmeler and A. Leri Aging and Disease as Modifiers of Efficacy of Cell Therapy Circ. Res., June 6, 2008; 102(11): 1319 - 1330. [Abstract] [Full Text] [PDF] A. Zampetaki, J. P. Kirton, and Q. Xu Vascular repair by endothelial progenitor cells Cardiovasc Res, June 1, 2008; 78(3): 413 - 421. [Abstract] [Full Text] [PDF]

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