SEARCH:   Advanced

Blood, 1 October 2007, Vol. 110, No. 7, pp. 2399-2407. Prepublished online as a Blood First Edition Paper on June 22, 2007; DOI 10.1182/blood-2006-10-051276. This Article Full Text (PDF) Supplemental Figure All Versions of this Article: blood-2006-10-051276v1 110/7/2399    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Qian, H. Articles by Ekblom, M. Search for Related Content PubMed PubMed Citation Articles by Qian, H. Articles by Ekblom, M. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted October 10, 2006 Accepted June 15, 2007 Distinct roles of integrins 6 and 4 in homing of fetal liver hematopoietic stem and progenitor cells Hong Qian, Elisabeth Georges-Labouesse, Alexander Nystrom, Anna Domogatskaya, Karl Tryggvason, Sten Eirik W Jacobsen, and Marja Ekblom* Hematopoietic Stem Cell Laboratory, Lund Strategic Center for Stem Cell Biology and Cell Therapy, Lund University, Lund, Sweden IGBMC, CNRS/INSERM/ULP, Illkirch, France Department of Cell and Matrix Biology, Lund University, Lund, Sweden Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden Department of Hematology, Lund University Hosptial, Lund, Sweden * Corresponding author; email: marja.ekblom{at}med.lu.se. Homing of hematopoietic stem cells (HSCs) into the bone marrow (BM) is a prerequisite for establishment of hematopoiesis during development and following transplantation. However, the molecular interactions that control homing of HSCs, in particular, of fetal HSCs, are not well understood. Herein, we studied the role of the 6 and 4 integrin receptors for homing and engraftment of fetal liver (FL) HSCs and hematopoietic progenitor cells (HPCs) to adult BM by using integrin 6 gene-deleted mice and function-blocking antibodies. Both integrins were ubiquitously expressed in FL Lin-Sca-1+Kit+ (LSK) cells. Deletion of integrin 6 receptor or inhibition by a function-blocking antibody inhibited FL LSK cell adhesion to its extracellular ligands, laminins-411 and -511 in vitro, and significantly reduced homing of HPCs to BM. In contrast, the anti-integrin 6 antibody did not inhibit BM homing of HSCs. In agreement with this, integrin 6 gene-deleted FL HSCs did not either display any homing or engraftment defect compared to wild-type littermates. In contrast, inhibition of integrin 4 receptor by a function-blocking antibody virtually abrogated homing of both FL HSCs and HPCs to BM, indicating distinct functions for integrin 6 and 4 receptors during homing of fetal HSCs and HPCs. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. D. Schreiber, C. Steinl, M. Essl, H. Abele, K. Geiger, C. A. Muller, W. K. Aicher, and G. Klein The integrin {alpha}9{beta}1 on hematopoietic stem and progenitor cells: involvement in cell adhesion, proliferation and differentiation Haematologica, November 1, 2009; 94(11): 1493 - 1501. [Abstract] [Full Text] [PDF] M. O. Ports, R. B. Nagle, G. D. Pond, and A. E. Cress Extracellular Engagement of {alpha}6 Integrin Inhibited Urokinase-Type Plasminogen Activator-Mediated Cleavage and Delayed Human Prostate Bone Metastasis Cancer Res., June 15, 2009; 69(12): 5007 - 5014. [Abstract] [Full Text] [PDF]

	Copyright © 2007 by American Society of Hematology         Online ISSN: 1528-0020