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Blood, 15 April 2007, Vol. 109, No. 8, pp. 3139-3146. Prepublished online as a Blood First Edition Paper on December 7, 2006December 14, 2006; DOI 10.1182/blood-2006-10-052787. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2006-10-052787v1 blood-2006-10-052787v2 109/8/3139    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Leon-Ponte, M. Articles by O'Connell, P. J Search for Related Content PubMed PubMed Citation Articles by Leon-Ponte, M. Articles by O'Connell, P. J Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted October 18, 2006 Accepted November 28, 2006 Serotonin provides an accessory signal to enhance T cell activation by signaling through the 5-HT7 receptor Matilde Leon-Ponte, Gerard P Ahern, and Peta J O'Connell* The Robarts Research Institute, London, Ontario, Canada Department of Pharmacology, Georgetown University, Washington, DC, United States Departments of Surgery, Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada * Corresponding author; email: peta{at}robarts.ca. Although typically considered a neurotransmitter, there is substantial evidence that serotonin (5-HT) plays an important role in the pathogenesis of inflammatory disorders. Despite these findings, the precise role of 5-HT in modulating immune function, particularly T cell function, remains elusive. We report that naive T cells predominantly express the type 7 5-HT receptor (5-HTR) and expression of this protein is substantially enhanced upon T cell activation. In addition, T cell activation leads to expression of the 5-HT1B and 5- HT2A receptors. Significantly, exogenous 5- HT induces rapid phosphorylation of extracellular signal- regulated kinase (ERK) 1/2 and IB in naive T cells. 5-HT-induced activation of ERK 1/2 and NFB is inhibited by preincubation with a specific 5-HT7 receptor antagonist. Thus, 5- HT signaling via the 5-HT7 receptor may contribute to early T cell activation. In turn, 5-HT synthesized by T cells may act as autocrine factor. Consistent with this hypothesis, we found that inhibition of 5-HT synthesis with parachlorophenylalanine (PCPA) impairs T cell activation and proliferation. Combined, these data demonstrate a fundamental role for 5-HT as an intrinsic co-factor in T cell activation and function, and suggest an alternative mechanism through which immune function is regulated by indoleamine 2,3-dioxygenase-mediated catabolism of tryptophan. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Serotonin: a real blast for T cells John Gordon and Nicholas M. Barnes Blood 2007 109: 3130-3131. [Full Text] [PDF] This article has been cited by other articles: S. C. Bischoff, R. Mailer, O. Pabst, G. Weier, W. Sedlik, Z. Li, J. J. Chen, D. L. Murphy, and M. D. Gershon Role of serotonin in intestinal inflammation: knockout of serotonin reuptake transporter exacerbates 2,4,6-trinitrobenzene sulfonic acid colitis in mice Am J Physiol Gastrointest Liver Physiol, March 1, 2009; 296(3): G685 - G695. [Abstract] [Full Text] [PDF] A Kling, M Seddighzadeh, L Arlestig, L Alfredsson, S Rantapaa-Dahlqvist, and L Padyukov Genetic variations in the serotonin 5-HT2A receptor gene (HTR2A) are associated with rheumatoid arthritis Ann Rheum Dis, August 1, 2008; 67(8): 1111 - 1115. [Abstract] [Full Text] [PDF]

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