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Blood, 1 June 2007, Vol. 109, No. 11, pp. 4777-4785. Prepublished online as a Blood First Edition Paper on February 8, 2007; DOI 10.1182/blood-2006-10-053280. This Article Full Text (PDF) Supplemental Figures and Tables All Versions of this Article: blood-2006-10-053280v1 109/11/4777    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Amatschek, S. Articles by Maurer, D. Search for Related Content PubMed PubMed Citation Articles by Amatschek, S. Articles by Maurer, D. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted October 19, 2006 Accepted February 3, 2007 Blood and lymphatic endothelial cell-specific differentiation programs are stringently controlled by the tissue environment Stefan Amatschek, Ernst Kriehuber, Wolfgang Bauer, Barbel Reininger, Paul Meraner, Alois Wolpl, Norbert Schweifer, Christian Haslinger, Georg Stingl, and Dieter Maurer* CeMM-Research Center for Molecular Medicine, of the Austrian Academy of Sciences, Vienna, Austria Department of Dermatology, Division of Immunology, Allergy and Infectious Diseases, Medical University of Vienna, Vienna, Austria Laboratory for Medical Genetics, Martinsried, Germany Boehringer-Ingelheim Austria, Vienna, Austria * Corresponding author; email: dieter.maurer{at}meduniwien.ac.at. The discovery of marker proteins of human blood (BECs) and lymphatic endothelial cells (LECs) has allowed to isolate these cells. So far, efforts to unravel their transcriptional and functional programs made use of cultured cells only. Hence, it is unknown to which extent previously identified LEC- and BEC-specific programs are representative of the in vivo situation. Here, we define the human BEC- and LEC-specific in vivo transcriptomes by comparative genome-wide expression profiling of freshly isolated cutaneous EC subsets and of non-EC skin cells (fibroblasts, mast cells, dendritic cells, epithelial cells). Interestingly, the expression of most of the newly identified EC subset-discriminating genes depends strictly on the in vivo tissue environment as revealed by comparative analyses of freshly isolated and cultured EC subsets. The identified environment-dependent, EC subset-restricted gene expression regulates lineage fidelity, fluid exchange, and MHC class II-dependent antigen presentation. As an example for a BEC-restricted in vivo function, we show that non-activated BECs in situ, but not in vitro, assemble and display MHC class II protein complexes loaded with self peptides. Thus, our data demonstrate the key importance of using precisely defined native ECs for the global identification of in vivo relevant cell functions. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. A. Tomei, S. Siegert, M. R. Britschgi, S. A. Luther, and M. A. Swartz Fluid Flow Regulates Stromal Cell Organization and CCL21 Expression in a Tissue-Engineered Lymph Node Microenvironment J. Immunol., October 1, 2009; 183(7): 4273 - 4283. [Abstract] [Full Text] [PDF] S. Lee, J. Kang, J. Yoo, S. K. Ganesan, S. C. Cook, B. Aguilar, S. Ramu, J. Lee, and Y.-K. Hong Prox1 physically and functionally interacts with COUP-TFII to specify lymphatic endothelial cell fate Blood, February 19, 2009; 113(8): 1856 - 1859. [Abstract] [Full Text] [PDF] C. Schuster, C. Vaculik, C. Fiala, S. Meindl, O. Brandt, M. Imhof, G. Stingl, W. Eppel, and A. Elbe-Burger HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells J. Exp. Med., January 16, 2009; 206(1): 169 - 181. [Abstract] [Full Text] [PDF] H. Ichise, T. Ichise, O. Ohtani, and N. Yoshida Phospholipase C{gamma}2 is necessary for separation of blood and lymphatic vasculature in mice Development, January 15, 2009; 136(2): 191 - 195. [Abstract] [Full Text] [PDF] J. W. Shin, R. Huggenberger, and M. Detmar Transcriptional profiling of VEGF-A and VEGF-C target genes in lymphatic endothelium reveals endothelial-specific molecule-1 as a novel mediator of lymphangiogenesis Blood, September 15, 2008; 112(6): 2318 - 2326. [Abstract] [Full Text] [PDF] C. S. McKimmie, A. R. Fraser, C. Hansell, L. Gutierrez, S. Philipsen, L. Connell, A. Rot, M. Kurowska-Stolarska, P. Carreno, M. Pruenster, et al. Hemopoietic Cell Expression of the Chemokine Decoy Receptor D6 Is Dynamic and Regulated by GATA1 J. Immunol., September 1, 2008; 181(5): 3353 - 3363. [Abstract] [Full Text] [PDF]

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