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Blood, 1 November 2007, Vol. 110, No. 9, pp. 3438-3446. Prepublished online as a Blood First Edition Paper on May 24, 2007; DOI 10.1182/blood-2006-11-055566. This Article Full Text (PDF) Supplemental Methods, Tables, and Figures Supplemental Figures and Videos All Versions of this Article: blood-2006-11-055566v1 110/9/3438    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Beltrami, A. P. Articles by Beltrami, C. A. Search for Related Content PubMed PubMed Citation Articles by Beltrami, A. P. Articles by Beltrami, C. A. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted November 3, 2006 Accepted May 17, 2007 Multipotent cells can be generated in vitro from several adult human organs (heart, liver and bone marrow) Antonio Paolo Beltrami, Daniela Cesselli, Natascha Bergamin, Patrizia Marcon, Silvia Rigo, Elisa Puppato, Federica D'Aurizio, Roberto Verardo, Silvano Piazza, Angela Pignatelli, Alessandra Poz, Umberto Baccarani, Daniela Damiani, Renato Fanin, Laura Mariuzzi, Nicoletta Finato, Paola Masolini, Silvia Burelli, Ottorino Belluzzi, Claudio Schneider, and Carlo Alberto Beltrami* Centro Interdipartimentale Medicina Rigenerativa (CIME), University of Udine, Udine, Italy LNCIB, Laboratorio Internazionale Consorzio Interuniversitario Biotecnologie, Trieste, Italy Dip. Biologia, Sezione di Fisiologia e Biofisica, Universita di Ferrara, Ferrara, Italy * Corresponding author; email: beltrami{at}uniud.it. The aims of our study were to verify whether it was possible to generate in vitro, from different adult human tissues, a population of cells that behaved, in culture, as multipotent stem cells and if these latter shared common properties. To this purpose we grew and cloned finite cell lines obtained from adult human liver, heart and bone marrow and named them human Multipotent Adult Stem Cells (hMASCs). Cloned hMASCs, obtained from the three different tissues, expressed the pluripotent-state-specific transcription factors Oct-4, NANOG and REX1, displayed telomerase activity, and exhibited a wide range of differentiation potential, as shown both at a morphological and functional level. hMASCs maintained a human diploid DNA content, and shared a common gene expression signature, as compared to several somatic cell lines and irrespectively of the tissue of isolation. In particular, the pathways regulating stem cell self-renewal/maintenance, such as Wnt, Hedgehog and Notch, were transcriptionally active. Our findings demonstrate that we have optimized an in vitro protocol to generate and expand cells from multiple organs that could be induced to acquire morphological and functional features of mature cells even embryologically not related to the tissue of origin. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Life in plastic is fantastic Paolo Bianco Blood 2007 110: 3090. [Full Text] [PDF] This article has been cited by other articles: S. Morikawa, Y. Mabuchi, Y. Kubota, Y. Nagai, K. Niibe, E. Hiratsu, S. Suzuki, C. Miyauchi-Hara, N. Nagoshi, T. Sunabori, et al. Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow J. Exp. Med., October 26, 2009; 206(11): 2483 - 2496. [Abstract] [Full Text] [PDF] B. J. Gersh, R. D. Simari, A. Behfar, C. M. Terzic, and A. Terzic Cardiac Cell Repair Therapy: A Clinical Perspective Mayo Clin. Proc., October 1, 2009; 84(10): 876 - 892. [Abstract] [Full Text] [PDF] M. Z. Ratajczak, D.-M. Shin, and M. Kucia Very Small Embryonic/Epiblast-Like Stem Cells: A Missing Link To Support the Germ Line Hypothesis of Cancer Development? Am. J. Pathol., June 1, 2009; 174(6): 1985 - 1992. [Abstract] [Full Text] [PDF] D. Cesselli, A. P. Beltrami, S. Rigo, N. Bergamin, F. D'Aurizio, R. Verardo, S. Piazza, E. Klaric, R. Fanin, B. Toffoletto, et al. Multipotent Progenitor Cells Are Present in Human Peripheral Blood Circ. Res., May 22, 2009; 104(10): 1225 - 1234. [Abstract] [Full Text] [PDF] W. Wojakowski, M. Tendera, M. Kucia, E. Zuba-Surma, E. Paczkowska, J. Ciosek, M. Halasa, M. Krol, M. Kazmierski, P. Buszman, et al. Mobilization of bone marrow-derived Oct-4+ SSEA-4+ very small embryonic-like stem cells in patients with acute myocardial infarction. J. Am. Coll. Cardiol., January 6, 2009; 53(1): 1 - 9. [Abstract] [Full Text] [PDF] L. S. Hart and W. S. El-Deiry Invincible, but Not Invisible: Imaging Approaches Toward In Vivo Detection of Cancer Stem Cells J. Clin. Oncol., June 10, 2008; 26(17): 2901 - 2910. [Abstract] [Full Text] [PDF] C. A. Beltrami Life is plastic Blood, March 1, 2008; 111(5): 2942 - 2943. [Full Text] [PDF]

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