SEARCH:   Advanced

Blood, 1 October 2007, Vol. 110, No. 7, pp. 2708-2717. Prepublished online as a Blood First Edition Paper on July 3, 2007; DOI 10.1182/blood-2006-11-056101. This Article Full Text (PDF) Supplemental Videos All Versions of this Article: blood-2006-11-056101v1 110/7/2708    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zennadi, R. Articles by Telen, M. J. Search for Related Content PubMed PubMed Citation Articles by Zennadi, R. Articles by Telen, M. J. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted November 13, 2006 Accepted May 23, 2007 Epinephrine-induced activation of LW-mediated sickle cell adhesion and vaso-occlusion in vivo Rahima Zennadi, Benjamin J Moeller, Erin J Whalen, Milena Batchvarova, Ke Xu, Siqing Shan, Martha Delahunty, Mark W Dewhirst, and Marilyn J. Telen* Division of Hematology, & Duke Comprehensive Sickle Cell Center, Duke University Medical Center, Durham, NC, United States Department of Radiation Oncology, Duke University Medical Center, Durham, NC, United States Division of Cardiology, Department of Medicine, Duke University Medical Center, Durham, NC, United States * Corresponding author; email: telen002{at}mc.duke.edu. Sickle red cell (SS RBC) adhesion is believed to contribute to the process of vaso-occlusion in sickle cell disease (SCD). We previously found that the LW RBC adhesion receptor can be activated by epinephrine to mediate SS RBC adhesion to endothelial v3 integrin. To determine the contribution of LW activation to vaso-occlusive events in vivo, we investigated whether in vitro treatment of SS RBCs by epinephrine resulted in vaso-occlusion in intact microvasculature after RBC infusion into nude mice. Epinephrine enhanced human SS but not normal RBC adhesion to murine endothelial cells in vitro and to endothelium in vivo, promoting vaso-occlusion and RBC organ sequestration. Murine sickle RBCs also responded to epinephrine with increased adhesion to postcapillary endothelium in nude mice. Epinephrine-induced SS RBC adhesion, vaso-occlusion and RBC organ trapping could be prevented by the -adrenergic receptor (AR) antagonist, propranolol. Infusion of soluble recombinant LW also significantly reduced adhesion and vaso-occlusion. In addition, epinephrine-treated SS RBCs induced activation of murine leukocyte adhesion to endothelium as well. We conclude that LW activation by epinephrine via -AR stimulation can promote both SS RBC and leukocyte adhesion as well as vaso-occlusion, suggesting that both epinephrine and LW play potentially pathophysiological roles in SCD. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Johnson and M. J. Telen Adhesion molecules and hydroxyurea in the pathophysiology of sickle cell disease Haematologica, April 1, 2008; 93(4): 481 - 485. [Full Text] [PDF]

	Copyright © 2007 by American Society of Hematology         Online ISSN: 1528-0020