Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Future Articles
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
 Blood, 1 March 2008, Vol. 111, No. 5, pp. 2725-2732.
Prepublished online as a Blood First Edition Paper on December 21, 2007; DOI 10.1182/blood-2006-11-056242.

This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Table
Right arrow All Versions of this Article:
blood-2006-11-056242v1
111/5/2725    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ostad, M.
Right arrow Articles by Sundblad, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ostad, M.
Right arrow Articles by Sundblad, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Next Article next article arrow

Submitted November 6, 2006
Accepted December 3, 2007

Expansion of immunoglobulin autoreactive T-helper cells in multiple myeloma

Masih Ostad, Margareta Andersson, Astrid Gruber, and Anne Sundblad*

Department of Medicine, Division of Hematology, Karolinska Institutet and University Hospital, Stockholm, Sweden

* Corresponding author; email: anne.sundblad{at}ki.se.

Activation and expansion of T helper (Th)-cells followed by regulation of activation, are essential to the generation of immune responses while limiting concomitant autoreactivity. In order to characterize T cells reactive towards myeloma derived monoclonal immunoglobulin (mIg), an autologous co-culture assay for single-cell analysis of mIg-responding cells was developed. When cultured with dendritic cells loaded with mIg, CD4+ Th-cells from patients with multiple myeloma (MM) showed a proliferative MHC class II-dependent response. CD8+ T cell reactivity and Th1-activation were consistently low or absent and Th2- and regulatory cytokines were expressed. The presence of such non-Th1 CD4+ T cells in peripheral blood was independent of treatment status, while the frequencies of responding cells varied between patients and reached the same order of magnitude as those measured for tetanus toxoid-specific Th memory cells. Furthermore, investigations of T cell subpopulations indicated a possible regulatory role on the mIg-responsiveness mediated by suppressive CD25highFOXP3+CD4+ T cells.

It is proposed from the present results that a predominant in vivo activation of non-Th1 mIg-reactive CD4+ T cells constitute an Ig-dependent auto-regulatory mechanism in human MM, with possible tumour growth supporting or permissive effects.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Cancer Res.Home page
A. Corthay, K. U. Lundin, K. B. Lorvik, P. O. Hofgaard, and B. Bogen
Secretion of Tumor-Specific Antigen by Myeloma Cells Is Required for Cancer Immunosurveillance by CD4+ T Cells
Cancer Res., July 15, 2009; 69(14): 5901 - 5907.
[Abstract] [Full Text] [PDF]


click for free articles
home about blood authors subscriptions permissions advertising public access contact us
  Copyright © 2007 by American Society of Hematology         Online ISSN: 1528-0020