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Blood, 1 July 2007, Vol. 110, No. 1, pp. 99-106. Prepublished online as a Blood First Edition Paper on March 16, 2007; DOI 10.1182/blood-2006-11-059204. This Article Full Text (PDF) Supplemental Tables and Figures All Versions of this Article: blood-2006-11-059204v1 110/1/99    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Liu, Y. Articles by Wen, Z. Search for Related Content PubMed PubMed Citation Articles by Liu, Y. Articles by Wen, Z. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted November 22, 2006 Accepted March 9, 2007 The zebrafish udu gene encodes a novel nuclear factor and is essential for primitive erythroid cell development Yanmei Liu, Linsen Du, Motomi Osato, Eng Hui Teo, Feng Qian, Hao Jin, Fenghua Zhen, Jin Xu, Lin Guo, Honghui Huang, Jun Chen, Robert Geisler, Yun-Jin Jiang, Jinrong Peng, and Zilong Wen* Laboratory of Molecular & Developmental Immunology, Institute of Molecular and Cell Biology, Singapore Department of Biological Sciences, The National University of Singapore, Singapore Laboratory of molecular oncology, Institute of Molecular and Cell Biology, Singapore Functional Genomics Laboratory, Institute of Molecular and Cell Biology, Singapore Max Planck Institute fuer Entwicklungsbiologie, Tuebingen, Germany Laboratory of Developmental Signalling & Patterning, Institute of Molecular and Cell Biology, Singapore * Corresponding author; email: zilong{at}imcb.a-star.edu.sg. Hematopoiesis is a complex process which gives rise to all blood lineages in the course of an organism's lifespan. Yet the underlying molecular mechanism governing this process is not fully understood. Here we report the isolation and detailed study of a newly identified zebrafish ugly duckling (udu) mutant allele, udusq1. We show that loss-of-function mutation in the udu gene disrupts primitive erythroid cell proliferation and differentiation in a cell-autonomous manner, resulting in red blood cell (RBC) hypoplasia. Positional cloning reveals that the udu gene encodes a novel factor that contains two Paired Amphipathic -Helix like (PAH-L) repeats and a putative SW13, ADA2, N-Cor and TFIIIB like (SANT-L) domain. We further show that the Udu protein is predominantly localized in the nucleus and deletion of the putative SANT-L domain abolishes its function. Our study indicates that the Udu protein is very likely to function as a transcription modulator essential for the proliferation and differentiation of erythroid lineage. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C.-H. Lim, S.-W. Chong, and Y.-J. Jiang Udu Deficiency Activates DNA Damage Checkpoint Mol. Biol. Cell, October 1, 2009; 20(19): 4183 - 4193. [Abstract] [Full Text] [PDF] F. Guillem, S. Lawson, C. Kannengiesser, M. Westerman, C. Beaumont, and B. Grandchamp Two nonsense mutations in the TMPRSS6 gene in a patient with microcytic anemia and iron deficiency Blood, September 1, 2008; 112(5): 2089 - 2091. [Abstract] [Full Text] [PDF] D. Carradice and G. J. Lieschke Zebrafish in hematology: sushi or science? Blood, April 1, 2008; 111(7): 3331 - 3342. [Abstract] [Full Text] [PDF]

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