Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
 Blood, 15 July 2007, Vol. 110, No. 2, pp. 553-560.
Prepublished online as a Blood First Edition Paper on March 29, 2007; DOI 10.1182/blood-2006-11-059246.

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
blood-2006-11-059246v1
110/2/553    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tokumasa, N.
Right arrow Articles by Nakajima, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tokumasa, N.
Right arrow Articles by Nakajima, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Next Article next article arrow

Submitted November 22, 2006
Accepted March 26, 2007

Expression of Tyk2 in dendritic cells is required for IL-12, IL-23, and IFN-{gamma} production and the induction of Th1 cell differentiation

Naoki Tokumasa, Akira Suto, Shin-ichiro Kagami, Shunsuke Furuta, Koichi Hirose, Norihiko Watanabe, Yasushi Saito, Kazuya Shimoda, Itsuo Iwamoto, and Hiroshi Nakajima*

Department of Allergy and Clinical Immunology, Clinical Cell Biology, Chiba University, Chiba, Japan
Department of Molecular Genetics, Graduate School of Medicine, Chiba University, Chiba, Japan
Gastroenterogy and Hematology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan
Research Center for Allergy and Clinical Immunology, Asahi General Hospital, Chiba, Japan

* Corresponding author; email: nakajimh{at}faculty.chiba-u.jp.

It is well documented that dendritic cells (DCs), representative antigen-presenting cells, are important sources of Th1-promoting cytokines and are actively involved in the regulation of T helper cell differentiation. However, the intracellular event that regulates this process is still largely unknown. In this study, we examined the role of Tyk2, a JAK kinase that is involved in the signaling pathway under IL-12 and IL-23, in DC functions. While the differentiation and maturation of DCs was normal in Tyk2-deficient (Tyk2-/-) mice, IL-12-induced Stat4 phosphorylation was diminished in Tyk2-/- DCs. IL-12-induced IFN-{gamma} production was also significantly diminished in Tyk2-/- DCs to the similar levels in Stat4-/- DCs. Interestingly, Tyk2-/- DCs were defective in IL-12 and IL-23 production upon stimulation with CpG ODN. Furthermore, Tyk2-/- DCs were impaired in their ability for the induction of Th1 cell differentiation but not of Th2 cell differentiation. Taken together, these results indicate that the expression of Tyk2 in DCs is crucial for the production of Th1-promoting cytokines such as IL-12 and IFN-{gamma} from DCs and thereby for the induction of antigen-specific Th1 cell differentiation.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




click for free articles
home about blood authors subscriptions permissions advertising public access contact us
  Copyright © 2007 by American Society of Hematology         Online ISSN: 1528-0020