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Blood, 15 October 2007, Vol. 110, No. 8, pp. 2889-2898. Prepublished online as a Blood First Edition Paper on July 20, 2007; DOI 10.1182/blood-2007-01-066316. This Article Full Text (PDF) Supplemental Methods and Figures All Versions of this Article: blood-2007-01-066316v1 110/8/2889    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chen, Y. Articles by Yang, Y.-C. Search for Related Content PubMed PubMed Citation Articles by Chen, Y. Articles by Yang, Y.-C. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 4, 2007 Accepted July 18, 2007 Cited2 is required for normal hematopoiesis in the murine fetal liver Yu Chen, Peter Haviernik, Kevin D. Bunting, and Yu-Chung Yang* Department of Pharmacology, Case Western Reserve University School of Medicine, Cleveland, OH Department of Medicine, Division of Hematology/Oncology, Case Western Reserve University School of Medicine, Cleveland, OH Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine, Cleveland, OH Center for Stem Cell and Regenerative Medicine, Case Western Reserve University School of Medicine, Cleveland, OH * Corresponding author; email: yu-chung.yang{at}case.edu. Cited2 [cAMP-responsive element-binding protein (CBP)/p300-interacting transactivators with glutamic acid (E) and aspartic acid (D)-rich tail 2] is a newly identified transcriptional modulator. Knockout of the Cited2 gene results in embryonic lethality with embryos manifesting heart and neural tube defects. Cited2-/- fetal liver displayed significant reduction in the numbers of Lin-c-Kit+Sca-1+ cells, Lin-c-Kit+ cells and progenitor cells of different lineages. Fetal liver cells from Cited2-/- embryos gave rise to markedly reduced number of colonies in the colony-forming unit assay. Primary and secondary transplantation studies showed significantly compromised reconstitution of T lymphoid, B lymphoid and myeloid lineages in mice transplanted with Cited2-/- fetal liver cells. Competitive reconstitution experiments further showed that fetal liver HSC function is severely impaired due to Cited2 deficiency. Microarray analysis showed decreased expression of Wnt5a and a panel of myeloid molecular markers such as PRTN3, MPO, neutrophil elastase, Cathepsin G and eosinophil peroxidase in Cited2-/- fetal livers. Decreased expression of Bmi-1, Notch1, LEF-1, Mcl-1 and GATA2 was also observed in Cited2-/- lin-c-Kit+ cells. The present study uncovers for the first time a novel role of Cited2 in the maintenance of hematopoietic homeostasis during embryogenesis and thus provides new insights into the molecular regulation of hematopoietic development. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Cited2: master regulator of HSC function? Hal E. Broxmeyer Blood 2007 110: 2787-2788. [Full Text] [PDF] This article has been cited by other articles: Y. Chen, Y.-q. Doughman, S. Gu, A. Jarrell, S.-i. Aota, A. Cvekl, M. Watanabe, S. L. Dunwoodie, R. S. Johnson, V. van Heyningen, et al. Cited2 is required for the proper formation of the hyaloid vasculature and for lens morphogenesis Development, September 1, 2008; 135(17): 2939 - 2948. [Abstract] [Full Text] [PDF]

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