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Blood, 1 August 2007, Vol. 110, No. 3, pp. 913-920. Prepublished online as a Blood First Edition Paper on April 4, 2007; DOI 10.1182/blood-2007-01-068718. This Article Full Text (PDF) Supplemental Figure All Versions of this Article: blood-2007-01-068718v1 110/3/913    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Obata, K. Articles by Karasuyama, H. Search for Related Content PubMed PubMed Citation Articles by Obata, K. Articles by Karasuyama, H. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 18, 2007 Accepted April 3, 2007 Basophils are essential initiators of a novel type of chronic allergic inflammation Kazushige Obata, Kaori Mukai, Yusuke Tsujimura, Kenji Ishiwata, Yohei Kawano, Yoshiyuki Minegishi, Naohiro Watanabe, and Hajime Karasuyama* Department of Immune Regulation, Tokyo Medical and Dental University Graduate School, Tokyo, Japan Department of Tropical Medicine, The Jikei University School of Medicine, Tokyo, Japan * Corresponding author; email: karasuyama.mbch{at}tmd.ac.jp. Basophils represent less than 1% of peripheral blood leukocytes, and have often been considered as minor and possibly redundant circulating mast cells. The recent finding that basophils readily generate large quantities of Th2 cytokines such as IL-4 provided new insights into the possible role of basophils in allergic disorders and immunity to pathogens. However, in-depth studies on basophils, particularly their functions in vivo, have been hampered by the lack of appropriate animal models such as mutant animals deficient only in basophils. Here, we established a mAb that reacted with mouse basophils and depleted them when administered in vivo. The mAb treatment of mice did not show any significant effect on classical allergic reactions such as passive cutaneous anaphylaxis and contact hypersensitivity. In contrast, it completely abolished the development of IgE-mediated chronic allergic dermatitis that is characterized by massive eosinophil infiltration, even though basophils accounted for only ~2% of the infiltrates. The treatment during the progression of the dermatitis resulted in drastic reduction in numbers of infiltrating eosinophils and neutrophils, concomitantly with elimination of basophils from the skin legions. Thus, basophils play a pivotal role in the development of IgE-mediated chronic allergic inflammation, as an initiator rather than as an effector. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: What does a basophil do? Cem Akin Blood 2007 110: 790-791. [Full Text] [PDF] This article has been cited by other articles: T. Shen, S. Kim, J.-s. Do, L. Wang, C. Lantz, J. F. Urban, G. Le Gros, and B. Min T cell-derived IL-3 plays key role in parasite infection-induced basophil production but is dispensable for in vivo basophil survival Int. Immunol., July 15, 2008; (2008) dxn077v1. [Abstract] [Full Text] [PDF] T. Kojima, K. Obata, K. Mukai, S. Sato, T. Takai, Y. Minegishi, and H. Karasuyama Mast Cells and Basophils Are Selectively Activated In Vitro and In Vivo through CD200R3 in an IgE-Independent Manner J. Immunol., November 15, 2007; 179(10): 7093 - 7100. [Abstract] [Full Text] [PDF]

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