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Blood, 1 September 2007, Vol. 110, No. 5, pp. 1550-1558.
Prepublished online as a Blood First Edition Paper on May 14, 2007; DOI 10.1182/blood-2007-01-069229.
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Submitted January 19, 2007
Accepted May 2, 2007
Human CD4+CD25+ regulatory T cells: proteome analysis identifies galectin-10 as a novel marker essential for their anergy and suppressive function
Jan Kubach, Petra Lutter, Tobias Bopp, Sabine Stoll, Christian Becker, Eva Huter, Christoph Richter, Petra Weingarten, Tobias Warger, Jurgen Knop, Stefan Mullner, John Wijdenes, Hansjorg Schild, Edgar Schmitt, and Helmut Jonuleit*
Department of Dermatology, University of Mainz, Mainz, Germany
Protagen AG, Dortmund, Germany
Institute of Immunology, University of Mainz, Mainz, Germany
Diaclone SAS, Besancon Cedex, France
* Corresponding author; email: jonuleit{at}hautklinik.klinik.uni-mainz.de.
CD4+CD25+Foxp3+ regulatory T cells (CD25+ Tregs) direct the maintenance of immunological self-tolerance by active suppression of autoaggressive T cell populations. However, the molecules mediating the anergic state and regulatory function of CD25+ Tregs are still elusive. Using differential proteomics, we identified galectin-10, a member of the lectin family, as constitutively expressed in human CD25+ Tregs while nearly absent in resting and activated CD4+ T cells. These data were confirmed on mRNA and protein level. Single cell staining and flow cytometry showed a strictly intracellular expression of galectin-10 in CD25+ Tregs. Specific inhibition of galectin-10 restored the proliferative capacity of CD25+ Tregs and abrogated their suppressive function. Notably, first identified here as expressed in human T lymphocytes, galectin-10 is essential for the functional properties of CD25+ Tregs.
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