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Blood, 1 December 2007, Vol. 110, No. 12, pp. 4077-4085. Prepublished online as a Blood First Edition Paper on August 9, 2007; DOI 10.1182/blood-2007-02-073841. This Article Full Text (PDF) Supplementary Figure All Versions of this Article: blood-2007-02-073841v1 110/12/4077    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Klein, I. Articles by Crispe, I. N. Search for Related Content PubMed PubMed Citation Articles by Klein, I. Articles by Crispe, I. N. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 12, 2007 Accepted July 25, 2007 Kupffer cell heterogeneity: functional properties of bone marrow-derived and sessile hepatic macrophages Ingo Klein*, Judith C Cornejo, Noelle K Polakos, Beena John, Sherry A Wuensch, David J Topham, Robert H Pierce, and Ian Nicholas Crispe David H. Smith Center for Vaccine Biology and Immunology, Aab Institute for Biomedical Research, University of Rochester School of Medicine and Dentistry, Rochester, NY, United States Department of Pathology, University of Rochester School of Medicine and Dentistry, Rochester, NY, United States Department of General Surgery, University of Wurzburg, Wurzburg, Germany * Corresponding author; email: klein_i{at}chirurgie.uni-wuerzburg.de. Kupffer cells form a large intravascular macrophage bed in the liver sinusoids. The differentiation history and diversity of Kupffer cells is disputed; some studies argue that they are derived from blood monocytes, while others support a local origin from intra-hepatic precursor cells. In the present study, we used both flow cytometry and immunohistochemistry to distinguish two subsets of Kupffer cells, which were revealed in the context both of bone marrow transplantation, and of orthotopic liver transplantation. One subset was radiosensitive and rapidly replaced from hematogenous precursors, while the other was relatively radio-resistant and long-lived. Both were phagocytic, but only the former population was recruited into inflammatory foci in response to CD8+ T cell activation. We propose the name "sessile" for the radio-resistant Kupffer cells that do not participate in immuno-inflammatory reactions. However, we found no evidence that these sessile Kupffer cells arise from immature intra-hepatic precursors. Our conclusions resolve a long-standing controversy and explain how different experimental approaches may reveal one or both of these subsets. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. Syres, F. Harrison, M. Tadlock, J. V. Jester, J. Simpson, S. Roy, D. R. Salomon, and S. Cherqui Successful treatment of the murine model of cystinosis using bone marrow cell transplantation Blood, September 17, 2009; 114(12): 2542 - 2552. [Abstract] [Full Text] [PDF] J. Ward, J. A. Guthrie, M. B. Sheridan, S. Boyes, J. T. Smith, D. Wilson, J. I. Wyatt, D. Treanor, and P. J. Robinson Sinusoidal Obstructive Syndrome Diagnosed With Superparamagnetic Iron Oxide-Enhanced Magnetic Resonance Imaging in Patients With Chemotherapy-Treated Colorectal Liver Metastases J. Clin. Oncol., September 10, 2008; 26(26): 4304 - 4310. [Abstract] [Full Text] [PDF]

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