SEARCH:   Advanced

Blood, 1 December 2007, Vol. 110, No. 12, pp. 3862-3870. Prepublished online as a Blood First Edition Paper on August 16, 2007; DOI 10.1182/blood-2007-02-074245. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2007-02-074245v1 110/12/3862    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chappaz, S. Articles by Finke, D. Search for Related Content PubMed PubMed Citation Articles by Chappaz, S. Articles by Finke, D. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 15, 2007 Accepted August 11, 2007 Increased TSLP availability restores T and B cell compartments in adult IL-7 deficient mice S. Chappaz, L. Flueck, A. G. Farr, A. G. Rolink, and D. Finke* Department of Clinical and Biological Sciences, Center for Biomedicine, University of Basel, Basel, Switzerland Department of Biological Structure, University of Washington, Seattle, WA, United States * Corresponding author; email: daniela.finke{at}unibas.ch. Interleukin 7 (IL-7) plays a crucial role in adult lymphopoiesis, while in fetal life its effect can be partially compensated by TSLP. Whether adult hematopoietic progenitor cells are unresponsive to TSLP or whether TSLP is less available in adult microenvironments is still a matter of debate. Here, we show that increased TSLP availability through transgene (Tg) expression fully restored lymphopoiesis in IL-7 deficient mice: it rescued B cell development, increased thymic and splenic cellularities, restored double negative (DN) thymocytes, and T cell generation and all peripheral lymphoid compartments. Analysis of bone marrow chimeras demonstrated that hematopoietic progenitor cells from adult wild type mice efficiently differentiated towards B and T cell lineages in lethally irradiated IL-7 deficient mice provided TSLP Tg was expressed in these mice. In vitro, TSLP promoted the differentiation of uncommitted adult bone marrow progenitors towards B and T lineages and the further differentiation of DN1 and DN2 thymocytes. Altogether, our results show that adult hematopoietic cells are TSLP-responsive and that TSLP can sustain long-term adult lymphopoiesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: N. D. Huntington, V. Labi, A. Cumano, P. Vieira, A. Strasser, A. Villunger, J. P. Di Santo, and N. L. Alves Loss of the pro-apoptotic BH3-only Bcl-2 family member Bim sustains B lymphopoiesis in the absence of IL-7 Int. Immunol., June 1, 2009; 21(6): 715 - 725. [Abstract] [Full Text] [PDF] B. C. Taylor, C. Zaph, A. E. Troy, Y. Du, K. J. Guild, M. R. Comeau, and D. Artis TSLP regulates intestinal immunity and inflammation in mouse models of helminth infection and colitis J. Exp. Med., March 16, 2009; 206(3): 655 - 667. [Abstract] [Full Text] [PDF] Y. Rochman and W. J. Leonard The Role of Thymic Stromal Lymphopoietin in CD8+ T Cell Homeostasis J. Immunol., December 1, 2008; 181(11): 7699 - 7705. [Abstract] [Full Text] [PDF] M. Guimond, W. J. Leonard, R. Spolski, S. W. Rossi, R. G. Veenstra, G. A. Hollander, C. L. Mackall, and B. R. Blazar Thymic stromal lymphopoietin is not necessary or sufficient to mediate the thymopoietic effects of keratinocyte growth factor Blood, January 15, 2008; 111(2): 969 - 970. [Full Text] [PDF]

	Copyright © 2007 by American Society of Hematology         Online ISSN: 1528-0020