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Blood, 15 September 2007, Vol. 110, No. 6, pp. 1806-1813. Prepublished online as a Blood First Edition Paper on May 25, 2007; DOI 10.1182/blood-2007-02-075382. This Article Full Text (PDF) All Versions of this Article: blood-2007-02-075382v1 110/6/1806    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Shepherd, B. E Articles by Abkowitz, J. L. Search for Related Content PubMed PubMed Citation Articles by Shepherd, B. E Articles by Abkowitz, J. L. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 20, 2007 Accepted May 9, 2007 Hematopoietic stem cell behavior in non-human primates Bryan E Shepherd, Hans-Peter Kiem, Peter M Lansdorp, Cynthia E Dunbar, Geraldine Aubert, Andre LaRochelle, Ruth Seggewiss, Peter Guttorp, and Janis L. Abkowitz* Department of Biostatistics, Vanderbilt University, Nashville, TN, United States Department of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, WA, United States Terry Fox Laboratory, University of British Columbia, Vancouver, Canada NHLBI, NIH, Bethesda, MD, United States Department of Statistics, University of Washington, Seattle, WA, United States Division of Hematology/Medicine, University of Washington, Seattle, WA, United States * Corresponding author; email: janabk{at}u.washington.edu. Little is known about the behavior of hematopoietic stem cells (HSC) in primates, as direct observations and competitive repopulation assays are not feasible. Therefore, we used two different and independent experimental strategies: the tracking of transgene expression after retroviral-mediated gene transfer (n=11 baboons; n=7 rhesus macaques) and quantitation of the average telomere length of granulocytes (n=132 baboons; n=14 macaques), together with stochastic methods, to study HSC kinetics in vivo. The average replication rate for baboon HSC is once per 36 weeks using gene marking analyses and once per 23 weeks using telomere shortening analyses. Comparable results were derived from the macaque data. These rates are substantially slower than the average replication rates previously reported for HSC in mice (once per 2.5 weeks) and cats (once per 8.3 weeks). Since baboons and macaques live for 25-45 years, much longer than mice (approximately 2 years) and cats (12-18 years), we can compute that HSC undergo a relatively constant number (~80-200) of lifetime replications. Our data thus suggest that the self-renewal capacity of mammalian stem cells in vivo is defined and evolutionarily conserved. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. W. Nienhuis Development of gene therapy for blood disorders Blood, May 1, 2008; 111(9): 4431 - 4444. [Abstract] [Full Text] [PDF] P. M. Lansdorp Telomeres, stem cells, and hematology Blood, February 15, 2008; 111(4): 1759 - 1766. [Abstract] [Full Text] [PDF]

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