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Blood, 15 September 2007, Vol. 110, No. 6, pp. 2201-2208. Prepublished online as a Blood First Edition Paper on June 14, 2007; DOI 10.1182/blood-2007-04-083097. This Article Full Text (PDF) All Versions of this Article: blood-2007-04-083097v1 110/6/2201    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zimring, J. C. Articles by Hillyer, C. D. Search for Related Content PubMed PubMed Citation Articles by Zimring, J. C. Articles by Hillyer, C. D. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 2, 2007 Accepted June 1, 2007 Non-hemolytic antigen-loss from RBCs requires cooperative binding of multiple antibodies recognizing different epitopes James C. Zimring*, Chantel M. Cadwell, Traci E. Chadwick, Steven L. Spitalnik, David A. Schirmer, Tao Wu, Charles A. Parkos, and Christopher D. Hillyer Center for Transfusion and Cellular Therapies, Dept of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, United States Dept of Pathology, College of Physicians & Surgeons, Columbia University, New York, NY, United States Epithelial Pathology Research Unit, Dept of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, United States * Corresponding author; email: jzimrin{at}emory.edu. Transfusion of crossmatch incompatible RBCs can result in antibody mediated hemolysis. However, in some patients, crossmatch incompatible RBCs lose the incompatible antigen from their surface, and then circulate normally ("antigen-loss"). Although antigen-loss has been reported in the settings of autoimmune hemolytic anemia and transfusion of crossmatch incompatible RBCs, mechanistic understanding of this phenomenon is limited. Using an in vivo murine model of antigen-loss, we report that, unlike polyclonal antisera, monoclonal antibodies did not induce antigen-loss. However, the combination of two monoclonal antibodies that recognized separate epitopes on the same antigen induced antigen-loss. This was not due to an increased number of Fc domains bound to the cell surface, as antigen-loss still occurred when combining intact monoclonal IgG and F(ab')2 fragments recognizing different epitopes. Together, these data lead to the hypothesis that antigen-antibody crosslinking is required for nonhemolytic antigen-loss to occur. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: E. A. Hod, C. M. Cadwell, J. S. Liepkalns, J. C. Zimring, S. A. Sokol, D. A. Schirmer, J. Jhang, and S. L. Spitalnik Cytokine storm in a mouse model of IgG-mediated hemolytic transfusion reactions Blood, August 1, 2008; 112(3): 891 - 894. [Abstract] [Full Text] [PDF]

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