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Blood, 1 December 2007, Vol. 110, No. 12, pp. 3917-3925. Prepublished online as a Blood First Edition Paper on July 20, 2007; DOI 10.1182/blood-2007-05-087767. This Article Full Text (PDF) All Versions of this Article: blood-2007-05-087767v1 110/12/3917    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jackson, S. M. Articles by Capra, J D. Search for Related Content PubMed PubMed Citation Articles by Jackson, S. M. Articles by Capra, J D. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted May 14, 2007 Accepted July 3, 2007 CD45RO enriches for activated, highly mutated human germinal center B cells Stephen M. Jackson, Natessa Harp, Darshna Patel, Jeffrey Zhang, Savannah Willson, Yoon J. Kim, Christian Clanton, and J Donald Capra* Molecular Immunogenetics Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, United States * Corresponding author; email: jdonald-capra{at}omrf.ouhsc.edu. To date, there is no consensus regarding the influence of different CD45 isoforms during peripheral B cell development. Examining correlations between surface CD45RO expression and various physiological processes ongoing during the germinal center reaction, we hypothesized that GC B cells, like T cells, that upregulate surface RO should progressively acquire phenotypes commonly associated with activated, differentiating lymphocytes. GC B cells (IgD-CD38+) were subdivided into three surface CD45RO fractions; RO-, RO+/-, and RO+. We show here that the average number of mutations per IgVH transcript increased in direct correlation with surface RO levels. Conjunctional use of RO and CD69 further delineated low/moderately and highly mutated fractions. AID mRNA was slightly reduced among RO+ GC B cells suggesting that higher mutation averages are unlikely due to elevated somatic mutation activity. Instead, RO+ GC B cells were negative for annexin V, comprised mostly (93%) of CD77- centrocytes, and were enriched for CD69+ cells. Collectively, RO+ GC B cells occupy what seems to be a specialized niche comprised mostly of centrocytes that may be in transition between activation states. These findings are among the first to sort GC B cells into populations enriched for live mutated cells solely using a single extracellular marker. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Gating on germinal center B cells Maurizio Zanetti Blood 2007 110: 3816-3817. [Full Text] [PDF] This article has been cited by other articles: S. M. Jackson, N. Harp, D. Patel, J. Wulf, E. D. Spaeth, U. K. Dike, J. A. James, and J. D. Capra Key developmental transitions in human germinal center B cells are revealed by differential CD45RB expression Blood, April 23, 2009; 113(17): 3999 - 4007. [Abstract] [Full Text] [PDF]

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