SEARCH:   Advanced

Blood, 15 January 2008, Vol. 111, No. 2, pp. 776-784. Prepublished online as a Blood First Edition Paper on September 21, 2007; DOI 10.1182/blood-2007-05-088310. This Article Full Text (PDF) Supplemental Tables and Figures All Versions of this Article: blood-2007-05-088310v1 111/2/776    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kawamata, N. Articles by Koeffler, H P. Search for Related Content PubMed PubMed Citation Articles by Kawamata, N. Articles by Koeffler, H P. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted May 2, 2007 Accepted September 13, 2007 Molecular allelokaryotyping of pediatric acute lymphoblastic leukemias by high resolution single nucleotide polymorphism oligonucleotide genomic microarray Norihiko Kawamata*, Seishi Ogawa, Martin Zimmermann, Motohiro Kato, Masashi Sanada, Kari Hemminki, Go Yamatomo, Yasuhito Nannya, Rolf Koehler, Thomas Flohr, Carl W Miller, Jochen Harbott, Wolf-Dieter Ludwig, Martin Stanulla, Martin Schrappe, Claus R Bartram, and H Phillip Koeffler Hematology/Oncology, Cedars-Sinai Medical Center/UCLA School of Medicine, Los Angeles, CA, United States Regeneration Medicine of Hematopoiesis, University of Tokyo, School of Medicine, Tokyo, Japan Department of Pediatric Hematology and Oncology, Children's Hospital, Hannover Medical School (MHH), Hannover, Germany Division of Molecular Genetic Epidemiology, German Cancer Research Center (DKFZ), Heidelberg, Germany Institute of Human Genetics, University of Heidelberg, Heidelberg, Germany Department of Pediatric Hematology and Oncology, Justus Liebig University, Gießen, Germany Department of Hematology, Oncology and Tumor Immunology, Robert-Rossle-Clinic at the HELIOS-Clinic Berlin-Buch, Charite, Berlin, Germany Department of Pediatrics, University of Kiel, Kiel, Germany * Corresponding author; email: kawamatan{at}cshs.org. Pediatric acute lymphoblastic leukemia (ALL) is a malignant disease resulting from accumulation of genetic alterations. A robust technology, single nucleotide polymorphism oligonucleotide genomic microarray (SNP-chip) in concert with bioinformatics offers the opportunity to discover the genetic lesions associated with ALL. We examined 399 pediatric ALL samples and their matched remissiom marrows at 50,000/ 250,000 SNP sites using a SNP-chip platform. Correlations between genetic abnormalities and clinical features were examined. Three common genetic alterations were found: deletion of ETV6, deletion of p16INK4A and hyperdiploidy, as well as, a number of novel recurrent genetic alterations. Uniparental disomy (UPD) was a frequent event, especially affecting chromosome 9. A cohort of children with hyperdiploid ALL without gain of chromosomes 17 and 18 had a poor prognosis. Molecular allelokaryotyping is a robust tool to define small genetic abnormalities including UPD which is usually overlooked by standard methods. This technique was able to detect subgroups with a poor prognosis based on their genetic status. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: A FISH 'n chips appetizer Beverly Lange Blood 2008 111: 480-481. [Full Text] [PDF] This article has been cited by other articles: T. Akagi, L.-Y. Shih, S. Ogawa, J. Gerss, S. R. Moore, R. Schreck, N. Kawamata, D.-C. Liang, M. Sanada, Y. Nannya, et al. Single nucleotide polymorphism genomic arrays analysis of t(8;21) acute myeloid leukemia cells Haematologica, September 1, 2009; 94(9): 1301 - 1306. [Abstract] [Full Text] [PDF] D. French, W. Yang, C. Cheng, S. C. Raimondi, C. G. Mullighan, J. R. Downing, W. E. Evans, C.-H. Pui, and M. V. Relling Acquired variation outweighs inherited variation in whole genome analysis of methotrexate polyglutamate accumulation in leukemia Blood, May 7, 2009; 113(19): 4512 - 4520. [Abstract] [Full Text] [PDF] D. Nowak, D. Stewart, and H. P. Koeffler Differentiation therapy of leukemia: 3 decades of development Blood, April 16, 2009; 113(16): 3655 - 3665. [Abstract] [Full Text] [PDF] D. Nowak, E. Le Toriellec, M.-H. Stern, N. Kawamata, T. Akagi, M. J. Dyer, W.-K. Hofmann, S. Ogawa, and H. P. Koeffler Molecular allelokaryotyping of T-cell prolymphocytic leukemia cells with high density single nucleotide polymorphism arrays identifies novel common genomic lesions and acquired uniparental disomy Haematologica, April 1, 2009; 94(4): 518 - 527. [Abstract] [Full Text] [PDF] S. Bea, I. Salaverria, L. Armengol, M. Pinyol, V. Fernandez, E. M. Hartmann, P. Jares, V. Amador, L. Hernandez, A. Navarro, et al. Uniparental disomies, homozygous deletions, amplifications, and target genes in mantle cell lymphoma revealed by integrative high-resolution whole-genome profiling Blood, March 26, 2009; 113(13): 3059 - 3069. [Abstract] [Full Text] [PDF] T. Akagi, L.-Y. Shih, M. Kato, N. Kawamata, G. Yamamoto, M. Sanada, R. Okamoto, C. W. Miller, D.-C. Liang, S. Ogawa, et al. Hidden abnormalities and novel classification of t(15;17) acute promyelocytic leukemia (APL) based on genomic alterations Blood, February 19, 2009; 113(8): 1741 - 1748. [Abstract] [Full Text] [PDF] T. Akagi, S. Ogawa, M. Dugas, N. Kawamata, G. Yamamoto, Y. Nannya, M. Sanada, C. W. Miller, A. Yung, S. Schnittger, et al. Frequent genomic abnormalities in acute myeloid leukemia/myelodysplastic syndrome with normal karyotype Haematologica, February 1, 2009; 94(2): 213 - 223. [Abstract] [Full Text] [PDF] C. G. Mullighan, X. Su, J. Zhang, I. Radtke, L. A.A. Phillips, C. B. Miller, J. Ma, W. Liu, C. Cheng, B. A. Schulman, et al. Deletion of IKZF1 and Prognosis in Acute Lymphoblastic Leukemia N. Engl. J. Med., January 29, 2009; 360(5): 470 - 480. [Abstract] [Full Text] [PDF] S. Sulong, A. V. Moorman, J. A. E. Irving, J. C. Strefford, Z. J. Konn, M. C. Case, L. Minto, K. E. Barber, H. Parker, S. L. Wright, et al. A comprehensive analysis of the CDKN2A gene in childhood acute lymphoblastic leukemia reveals genomic deletion, copy number neutral loss of heterozygosity, and association with specific cytogenetic subgroups Blood, January 1, 2009; 113(1): 100 - 107. [Abstract] [Full Text] [PDF] J. L. Wiemels, J. Hofmann, M. Kang, R. Selzer, R. Green, M. Zhou, S. Zhong, L. Zhang, M. T. Smith, C. Marsit, et al. Chromosome 12p Deletions in TEL-AML1 Childhood Acute Lymphoblastic Leukemia Are Associated with Retrotransposon Elements and Occur Postnatally Cancer Res., December 1, 2008; 68(23): 9935 - 9944. [Abstract] [Full Text] [PDF] C. S. Walsh, S. Ogawa, D. R. Scoles, C. W. Miller, N. Kawamata, S. A. Narod, H. P. Koeffler, and B. Y. Karlan Genome-Wide Loss of Heterozygosity and Uniparental Disomy in BRCA1/2-Associated Ovarian Carcinomas Clin. Cancer Res., December 1, 2008; 14(23): 7645 - 7651. [Abstract] [Full Text] [PDF] Q. An, S. L. Wright, Z. J. Konn, E. Matheson, L. Minto, A. V. Moorman, H. Parker, M. Griffiths, F. M. Ross, T. Davies, et al. Variable breakpoints target PAX5 in patients with dicentric chromosomes: A model for the basis of unbalanced translocations in cancer PNAS, November 4, 2008; 105(44): 17050 - 17054. [Abstract] [Full Text] [PDF] N. Kawamata, S. Ogawa, M. Zimmermann, B. Niebuhr, C. Stocking, M. Sanada, K. Hemminki, G. Yamatomo, Y. Nannya, R. Koehler, et al. Cloning of genes involved in chromosomal translocations by high-resolution single nucleotide polymorphism genomic microarray PNAS, August 19, 2008; 105(33): 11921 - 11926. [Abstract] [Full Text] [PDF] J. P. Maciejewski and G. J. Mufti Whole genome scanning as a cytogenetic tool in hematologic malignancies Blood, August 15, 2008; 112(4): 965 - 974. [Abstract] [Full Text] [PDF] K. Paulsson, J.-B. Cazier, F. MacDougall, J. Stevens, I. Stasevich, N. Vrcelj, T. Chaplin, D. M. Lillington, T. A. Lister, and B. D. Young Microdeletions are a general feature of adult and adolescent acute lymphoblastic leukemia: Unexpected similarities with pediatric disease PNAS, May 6, 2008; 105(18): 6708 - 6713. [Abstract] [Full Text] [PDF]

	Copyright © 2007 by American Society of Hematology         Online ISSN: 1528-0020