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Blood, 1 November 2007, Vol. 110, No. 9, pp. 3150-3157.
Prepublished online as a Blood First Edition Paper on August 6, 2007; DOI 10.1182/blood-2007-05-092262.
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Submitted May 25, 2007
Accepted July 30, 2007
Human BSAP and BLIMP1 conform an autoregulatory feedback loop
Francisco Mora-Lopez, Elena Reales, Jose A Brieva, and Antonio Campos-Caro*
Unidad de Investigacion, Hospital Universitario Puerta del Mar, Cadiz, Spain
* Corresponding author; email: antonio.campos.exts{at}juntadeandalucia.es.
B lymphocyte-induced maturation protein-1 (BLIMP1), encoded by the PRDM1 gene, is a transcriptional repressor considered a master regulator that is required and sufficient for plasma cell (PC) differentiation. BLIMP1 represses the PAX5 gene, coding for the B-cell lineage-specific activator protein (BSAP), which is required for B-cell identity and survival. Mutations in PAX5 gene as well as in PRDM1 gene have been recently implicated in lymphomas. In the present study, sequence analysis of PRDM1 gene revealed a binding site for BSAP transcription factor. By analyzing different human cell lines, we have found that a specific nuclear factor for B cell lines binds to a site on the PRDM1 promoter. Electrophoretic mobility shift assays identified this factor as BSAP, and chromatin immunoprecipitation assays confirmed its binding in vivo to the human PRDM1 promoter. Moreover, by ectopically expressing BSAP, and using a PRDM1 promoter with the BSAP binding site mutated, we demonstrated that this factor represses the expression of BLIMP1. Therefore, repression of PRDM1 by BSAP reveals an autoregulatory negative feedback loop that could play a relevant role in controlling human PC differentiation.
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