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Blood, 15 January 2008, Vol. 111, No. 2, pp. 588-595. Prepublished online as a Blood First Edition Paper on September 28, 2007; DOI 10.1182/blood-2007-05-092718. This Article Full Text (PDF) All Versions of this Article: blood-2007-05-092718v1 111/2/588    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wang, Q. Articles by Chen, Y.-G. Search for Related Content PubMed PubMed Citation Articles by Wang, Q. Articles by Chen, Y.-G. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted May 31, 2007 Accepted September 24, 2007 MicroRNA miR-24 inhibits erythropoiesis by targeting activin type I receptor ALK4 Qiang Wang, Zheng Huang, Huiling Xue, Chengcheng Jin, Xiu-Li Ju, Jing-Dong J Han, and Ye-Guang Chen* State Key Laboratory of Biomembrane & Membrane Biotechnology, Dept of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China Center for Molecular Systems Biology, Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing, China Department of Pediatrics, Qilu Hospital of Shandong University, Jinan, China * Corresponding author; email: ygchen{at}tsinghua.edu.cn. MicroRNAs have been suggested to modulate a variety of cellular events. Here we report that miR-24 regulates erythroid differentiation by influencing the expression of human activin type I receptor ALK4 (hALK4). Ectopic expression of miR-24 reduces the mRNA and protein levels of hALK4 by targeting the 3'-untranslated region of hALK4 mRNA and interferes with activin-induced Smad2 phosphorylation and reporter expression. Furthermore, miR-24 represses the activin-mediated accumulation of hemoglobin, an erythroid differentiation marker, in erythroleukemic K562 cells and decreases erythroid colony-forming and burst-forming units of CD34+ hematopoietic progenitor cells. ALK4 expression is inversely correlated with miR-24 expression during the early stages of erythroid differentiation, and the forced expression of miR-24 leads to a delay of activin-induced maturation of hematopoietic progenitor cells in liquid culture. Thus, our findings define a regulation mode of miR-24 on erythropoiesis by impeding ALK4 expression. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. Yeligar, H. Tsukamoto, and V. K. Kalra Ethanol-Induced Expression of ET-1 and ET-BR in Liver Sinusoidal Endothelial Cells and Human Endothelial Cells Involves Hypoxia-Inducible Factor-1{alpha} and MicroRNA-199 J. Immunol., October 15, 2009; 183(8): 5232 - 5243. [Abstract] [Full Text] [PDF] N. Felli, F. Pedini, P. Romania, M. Biffoni, O. Morsilli, G. Castelli, S. Santoro, S. Chicarella, A. Sorrentino, C. Peschle, et al. MicroRNA 223-dependent expression of LMO2 regulates normal erythropoiesis Haematologica, April 1, 2009; 94(4): 479 - 486. [Abstract] [Full Text] [PDF] L. Pase, J. E. Layton, W. P. Kloosterman, D. Carradice, P. M. Waterhouse, and G. J. Lieschke miR-451 regulates zebrafish erythroid maturation in vivo via its target gata2 Blood, February 19, 2009; 113(8): 1794 - 1804. [Abstract] [Full Text] [PDF] Y.-F. Fu, T.-T. Du, M. Dong, K.-Y. Zhu, C.-B. Jing, Y. Zhang, L. Wang, H.-B. Fan, Y. Chen, Y. Jin, et al. Mir-144 selectively regulates embryonic {alpha}-hemoglobin synthesis during primitive erythropoiesis Blood, February 5, 2009; 113(6): 1340 - 1349. [Abstract] [Full Text] [PDF] J. Ho, K. H. Ng, S. Rosen, A. Dostal, R. I. Gregory, and J. A. Kreidberg Podocyte-Specific Loss of Functional MicroRNAs Leads to Rapid Glomerular and Tubular Injury J. Am. Soc. Nephrol., November 1, 2008; 19(11): 2069 - 2075. [Abstract] [Full Text] [PDF]

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