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Blood, 1 January 2008, Vol. 111, No. 1, pp. 351-358.
Prepublished online as a Blood First Edition Paper on September 26, 2007; DOI 10.1182/blood-2007-06-094151.
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Submitted June 12, 2007
Accepted September 17, 2007
Gcet1 (centerin), a highly restricted marker for a subset of Germinal Centre-derived lymphomas
Santiago Montes-Moreno*, Giovanna Roncador, Lorena Maestre, Nerea Martinez, Lydia Sanchez-Verde, Francisca Camacho, Jimena Cannata, Jorge Luis Martinez-Torrecuadrada, Yulei Shen, Wing C Chan, and Miguel Angel Piris
Department of Pathology, Hospital Universitario 12 de Octubre, Madrid, Spain
Monoclonal Antibody Unit, Spanish National Cancer Centre, Madrid, Spain
Lymphoma Group, Spanish National Cancer Centre, Madrid, Spain
Histology and Immunohistochemistry Unit, Spanish National Cancer Centre, Madrid, Spain
Protein Technology Unit, Spanish National Cancer Centre, Madrid, Spain
Department of Pathology and Microbiology, University of Nebraska Medical Centre, Omaha, NE, United States
* Corresponding author; email: smontes{at}cnio.es.
GCET1 (Germinal Centre B cell-Expressed Transcript-1) gene codes for a serpin expressed in Germinal Centre B-cells. Following the observation that Follicular Lymphoma cases exhibit an increased level of Gcet1 expression, when compared with follicular hyperplasia, we have characterized Gcet1 protein expression in human tissues, cell lines and a large series of lymphomas. To this end we have performed immunohistochemical and Western-Blot analyses using a newly generated monoclonal antibody that is reactive in paraffin-embedded tissues. Our results demonstrate that Gcet1 is expressed exclusively by neoplasms hypothetically to be arrested at the GC stage of differentiation, including FL, NLPHL and a subset of DLBCL, T/HRBCL and BL. Within these tumors, Gcet-1 protein expression is restricted to a subset of GC B cells, establishing the existence of a distinct heterogeneity among normal and neoplastic GC B cells. None of the other B-cell lymphomas, i.e., CLL, SMZL and MCL, was Gcet1-positive, which underlines the potential utility of Gcet1 expression in lymphoma diagnosis. The results of RNA and protein expression should prompt further investigation into the role of Gcet1 in regulating B cell survival.
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