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Blood, 1 January 2008, Vol. 111, No. 1, pp. 132-141. Prepublished online as a Blood First Edition Paper on September 17, 2007; DOI 10.1182/blood-2007-06-095398. This Article Full Text (PDF) Supplemental Figures and Videos All Versions of this Article: blood-2007-06-095398v1 111/1/132    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Le Guyader, D. Articles by Herbomel, P. Search for Related Content PubMed PubMed Citation Articles by Le Guyader, D. Articles by Herbomel, P. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted June 14, 2007 Accepted September 12, 2007 Origins and unconventional behavior of neutrophils in developing zebrafish Dorothee Le Guyader, Michael J Redd, Emma Colucci-Guyon, Emi Murayama, Karima Kissa, Valerie Briolat, Elodie Mordelet, Agustin Zapata, Hiroto Shinomiya, and Philippe Herbomel* Unite Macrophages et Developpement de l'Immunite, CNRS-URA 2578, Institut Pasteur, Paris, France Huntsman Cancer Institute, University of Utah, Salt Lake City, UT, United States Department of Cell Biology, Faculty of Biology, Complutense University, Madrid, Spain Department of Immunology and Host Defenses, Ehime University School of Medicine, Ehime, Japan * Corresponding author; email: herbomel{at}pasteur.fr. The first leukocytes that arise in the development of vertebrate embryos are the primitive macrophages, which differentiate in the yolk sac, and then quickly invade embryonic tissues. These macrophages have been considered to constitute a separate lineage giving rise to no other cell type. Using an in vivo photoactivatable cell tracer in the transparent zebrafish embryo, we demonstrate that this lineage also gives rise to an equal or higher number of neutrophilic granulocytes. Surprisingly, the differentiation of these primitive neutrophils occurs only after primitive myeloid progenitors have dispersed in the tissues. By 2 days post-fertilization, these neutrophils have become the major leukocyte type found wandering in the epidermis and mesenchyme. Like the primitive macrophages, all primitive and larval neutrophils express PU.1 and L-plastin, they are highly attracted to local infections, yet only a small fraction of them phagocytose microbes, and to a much lesser extent per cell than the macrophages. They are also attracted to variously stressed or malformed tissues, suggesting a wider role than anti-microbial defence. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: A photographic fishing expedition with granie Mitchell S. Cairo Blood 2008 111: 4-5. [Full Text] [PDF] This article has been cited by other articles: J.-P. Levraud, O. Disson, K. Kissa, I. Bonne, P. Cossart, P. Herbomel, and M. Lecuit Real-Time Observation of Listeria monocytogenes-Phagocyte Interactions in Living Zebrafish Larvae Infect. Immun., September 1, 2009; 77(9): 3651 - 3660. [Abstract] [Full Text] [PDF] C. Hall, M. V. Flores, A. Chien, A. Davidson, K. Crosier, and P. 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