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Blood, 1 February 2008, Vol. 111, No. 3, pp. 1700-1708. Prepublished online as a Blood First Edition Paper on November 9, 2007; DOI 10.1182/blood-2007-06-098178. This Article Full Text (PDF) Supplemental Figure All Versions of this Article: blood-2007-06-098178v1 111/3/1700    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Rhodes, M. M Articles by Koury, M. J Search for Related Content PubMed PubMed Citation Articles by Rhodes, M. M Articles by Koury, M. J Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted June 27, 2007 Accepted October 31, 2007 Adherence to macrophages in erythroblastic islands enhances erythroblast proliferation and increases erythrocyte production by a different mechanism than erythropoietin Melissa M Rhodes, Prapaporn Kopsombut, Maurice C Bondurant, James O Price, and Mark J Koury* Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, United States Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, United States Department of Pathology, VA Tennessee Valley Healthcare System & Vanderbilt University Medical Center, Nashville, TN, United States Department of Medicine, VA Tennessee Valley Healthcare System & Vanderbilt University Medical Center, Nashville, TN, United States * Corresponding author; email: mark.koury{at}vanderbilt.edu. Erythroblasts adhere to central macrophages forming erythroblastic islands in hematopoietic tissues, but the function of these islands is not understood. Murine erythroblastic islands were reconstituted in vitro with macrophages and developmentally synchronous proerythroblasts. Erythroblasts co-cultured with macrophages proliferated three-fold greater than erythroblasts cultured alone. Direct contact with the macrophages was necessary for this enhanced erythroblast proliferation, which resulted from decreased transit time in the G0/G1 phase of cell cycle. Increased erythroblast proliferation in erythroblastic islands occurred over a wide range of erythropoietin concentrations and was due to a different mechanism than the anti-apoptotic effect of erythropoietin. Erythroblasts adherent to macrophages had slightly delayed enucleation, but otherwise differentiation was similar to erythroblasts cultured alone or those that became nonadherent in co-cultures. These results suggest a mechanism for the development of anemias associated with abnormal macrophage function and for reduced responsiveness of those anemias to erythropoietin therapy. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. J. Brandt and M. J. Koury Regulation of LMO2 mRNA and protein expression in erythroid differentiation Haematologica, April 1, 2009; 94(4): 447 - 448. [Full Text] [PDF] J. A. Chasis and N. Mohandas Erythroblastic islands: niches for erythropoiesis Blood, August 1, 2008; 112(3): 470 - 478. [Abstract] [Full Text] [PDF] J. Isern, S. T. Fraser, Z. He, and M. H. Baron The fetal liver is a niche for maturation of primitive erythroid cells PNAS, May 6, 2008; 105(18): 6662 - 6667. [Abstract] [Full Text] [PDF] K. E. McGrath, P. D. Kingsley, A. D. Koniski, R. L. Porter, T. P. Bushnell, and J. Palis Enucleation of primitive erythroid cells generates a transient population of "pyrenocytes" in the mammalian fetus Blood, February 15, 2008; 111(4): 2409 - 2417. [Abstract] [Full Text] [PDF]

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