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Blood, 15 August 2008, Vol. 112, No. 4, pp. 1166-1174. Prepublished online as a Blood First Edition Paper on April 7, 2008; DOI 10.1182/blood-2007-06-098327. This Article Full Text (PDF) All Versions of this Article: blood-2007-06-098327v1 112/4/1166    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Xu, H. Articles by Liversidge, J. Search for Related Content PubMed PubMed Citation Articles by Xu, H. Articles by Liversidge, J. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted June 27, 2007 Accepted March 10, 2008 Critical but divergent roles for CD62L and CD44 in directing blood monocyte trafficking in vivo during inflammation Heping Xu, Ayyakkannu Manivannan, Isabel J Crane, Rosemary Dawson, and Janet Liversidge* Department of Ophthamology, University of Aberdeen Institute of Medical Sciences, Aberdeen, United Kingdom Department of Radiology, University of Aberdeen, Foresterhill, Aberdeen, United Kingdom * Corresponding author; email: j.liversidge{at}abdn.ac.uk. Using non-invasive in vivo imaging and experimental autoimmune uveoretinitis as a model we show for the first time, that the mechanisms controlling blood monocyte recirculation through peripheral and lymphoid tissues alter during inflammation. The recirculation of monocytes in mice with ocular inflammation, but not controls was found to be dependent upon CD62-L and CD44. Not only was rolling efficiency ablated or markedly reduced in antibody treated mice, the majority of labelled monocytes disappeared from the circulation within seconds, anti-CD44 treated monocytes homing to the lymph nodes and anti-CD62-L treated monocytes homing to the spleen. Our data indicate that while PSGL-1 has a partial role in the transmigration of monocytes into the inflamed retina, CD62-L has a key role in regulating recruitment of monocytes to lymphoid tissue from the blood during inflammation and that CD44 is required to maintain CD62-L+ve inflammatory monocytes within the circulation during inflammation. This effect was systemic, as sequestered monocytes accumulated in mesenteric as well as draining cervical lymph nodes, and inflammation dependent, as depletion of circulating blood monocytes was much reduced or absent in normal mice and accumulations of adoptively transferred monocytes in the lymphoid tissues did not occur. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Teasing out monocyte trafficking mechanisms Christopher V. Carman Blood 2008 112: 929-930. [Full Text] [PDF] This article has been cited by other articles: A. Dagkalis, C. Wallace, H. Xu, S. Liebau, A. Manivannan, M. A. Stone, M. Mack, J. Liversidge, and I. J. Crane Development of Experimental Autoimmune Uveitis: Efficient Recruitment of Monocytes Is Independent of CCR2 Invest. Ophthalmol. Vis. Sci., September 1, 2009; 50(9): 4288 - 4294. [Abstract] [Full Text] [PDF]

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