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Blood, 15 March 2008, Vol. 111, No. 6, pp. 3183-3189. Prepublished online as a Blood First Edition Paper on January 10, 2008; DOI 10.1182/blood-2007-07-098749. This Article Full Text (PDF) Supplemental Tables More Supplemental Tables and Supplemental Figures All Versions of this Article: blood-2007-07-098749v1 111/6/3183    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Garzon, R. Articles by Croce, C. M. Search for Related Content PubMed PubMed Citation Articles by Garzon, R. Articles by Croce, C. M. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 2, 2007 Accepted December 30, 2007 MicroRNA signatures associated with cytogenetics and prognosis in acute myeloid leukemia Ramiro Garzon, Stefano Volinia, Chang-Gong Liu, Cecilia Fernandez-Cymering, Tiziana Palumbo, Flavia Pichiorri, Muller Fabbri, Kevin Coombes, Hansjuerg Alder, Tatsuya Nakamura, Neal Flomenberg, Guido Marcucci, George A. Calin, Steven M. Kornblau, Hagop Kantarjian, Clara D. Bloomfield, Michael Andreeff, and Carlo M. Croce* Division of Hematology and Oncology, Department of Internal Medicine, Comprehensive Cancer Center, The Ohio State University, Columbus, OH, United States DAMA, Data Mining of DNA Microarrays, University of Ferrara, Ferrara, Italy Depament of Molecular Virology, Immunology and Medical Genetics, Comprehensive Cancer Center, The Ohio State University, Columbus, OH, United States Biostatistics Division, MD Anderson Cancer Center, Texas State University, Houston, TX, United States Department of Medicine, The Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, United States Experimental Therapeutics & Cancer Genetics, MD Anderson Cancer Center, Texas State University, Houston, TX, United States Department of Blood and Bone Marrow Transplantation, MD Anderson Cancer Center, Texas State University, Houston, TX, United States Department of Leukemia, MD Anderson Cancer Center, Texas State University, Houston, TX, United States * Corresponding author; email: carlo.croce{at}osumc.edu. MicroRNAs (miRNAs) are small RNAs of 19-25 nucleotides that are negative regulators of gene expression. To determine whether miRNAs are associated with cytogenetic abnormalities and clinical features in acute myeloid leukemia (AML), we evaluated the miRNA expression of CD34+ cells and 122 untreated adult AML cases using a microarray platform. After background subtraction and normalization using a set of housekeeping genes, data were analyzed using Significance Analysis of Microarrays. An independent set of 60 untreated AML patients was used to validate the outcome signatures using real time PCR. We identified several miRNAs differentially expressed between CD34+ normal cells and the AML samples. MiRNA expression was also closely associated with selected cytogenetic and molecular abnormalities like t(11q23), isolated trisomy 8 and FLT3-ITD mutations. Furthermore, patients with high expression of miR-191 and miR-199a had significantly worse overall and event free survival than AML patients with low expression (Overall survival: miR-191 P=0.03 and miR-199a P=0.0006, Cox regression). In conclusion, miRNA expression in AML is closely associated with cytogenetics and FLT3-ITD mutations. A small subset of miRNAs is correlated with survival. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. V. Iorio and C. M. Croce MicroRNAs in Cancer: Small Molecules With a Huge Impact J. Clin. Oncol., December 1, 2009; 27(34): 5848 - 5856. [Abstract] [Full Text] [PDF] A. Navarro, S. Bea, V. Fernandez, M. Prieto, I. Salaverria, P. Jares, E. Hartmann, A. Mozos, A. Lopez-Guillermo, N. Villamor, et al. 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[Abstract] [Full Text] [PDF] E. Bandres, N. Bitarte, F. Arias, J. Agorreta, P. Fortes, X. Agirre, R. Zarate, J. A. Diaz-Gonzalez, N. Ramirez, J. J. Sola, et al. microRNA-451 Regulates Macrophage Migration Inhibitory Factor Production and Proliferation of Gastrointestinal Cancer Cells Clin. Cancer Res., April 1, 2009; 15(7): 2281 - 2290. [Abstract] [Full Text] [PDF] M. Pigazzi, E. Manara, E. Baron, and G. Basso miR-34b Targets Cyclic AMP-Responsive Element Binding Protein in Acute Myeloid Leukemia Cancer Res., March 15, 2009; 69(6): 2471 - 2478. [Abstract] [Full Text] [PDF] J. Roman-Gomez, X. Agirre, A. Jimenez-Velasco, V. Arqueros, A. Vilas-Zornoza, P. Rodriguez-Otero, I. Martin-Subero, L. Garate, L. Cordeu, E. San Jose-Eneriz, et al. Epigenetic Regulation of MicroRNAs in Acute Lymphoblastic Leukemia J. Clin. Oncol., March 10, 2009; 27(8): 1316 - 1322. [Abstract] [Full Text] [PDF] C. Mascaux, J. F. Laes, G. Anthoine, A. Haller, V. Ninane, A. Burny, and J. P. 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