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Blood, 1 February 2008, Vol. 111, No. 3, pp. 1147-1156. Prepublished online as a Blood First Edition Paper on October 12, 2007; DOI 10.1182/blood-2007-07-099499. This Article Full Text (PDF) Supplemental Table and Figures Supplemental Videos Supplemental Videos All Versions of this Article: blood-2007-07-099499v1 111/3/1147    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kissa, K. Articles by Herbomel, P. Search for Related Content PubMed PubMed Citation Articles by Kissa, K. Articles by Herbomel, P. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 5, 2007 Accepted October 5, 2007 Live imaging of emerging hematopoietic stem cells and early thymus colonization Karima Kissa, Emi Murayama, Agustin Zapata, Alfonso Cortes, Emmanuelle Perret, Christophe Machu, and Philippe Herbomel* Unite Macrophages et Developpement de l'Immunite, CNRS-URA, Paris, France Department of Cell Biology, Faculty of Biology, Complutense University, Madrid, Spain Plate-forme d'Imagerie Dynamique, Institut Pasteur, Paris, France * Corresponding author; email: herbomel{at}pasteur.fr. We recently demonstrated in zebrafish the developmental migration of emerging hematopoietic stem cells (HSCs) that is thought to occur in mammalian embryos, from the Aorta-Gonad-Mesonephros (AGM) area to the successive hematopoietic organs. CD41 is the earliest known molecular marker of nascent HSCs in mammalian development. Here we show that in CD41-gfp transgenic zebrafish embryos, the transgene is expressed by emerging HSCs in the AGM, allowing for the first time to image their behavior and trace them in real-time. We find that the zebrafish AGM contains no intra-aortic cell clusters, so far considered a hallmark of HSC emergence. CD41-GFPlow HSCs emerge in the sub-aortic mesenchyme, and enter the circulation not through the dorsal aorta but through the axial vein, whose peculiar structure facilitates their intravasation. The rise in CD41-gfp expression among c-myb+ HSC precursors is asynchronous, and marks their competence to leave the AGM and immediately seed the Caudal Hematopoietic Tissue (which has a hematopoietic function analogous to the mammalian fetal liver). Imaging the later migration of CD41-GFP+ precursors to the nascent thymus reveals that although some reach the thymus by extravasating from the nearest vein, most travel for hours through the mesenchyme, from surprisingly diverse and remote sites of extravasation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Stem-cell trafficking at vascular borders Sarah Hutchinson and Nikolaus S. Trede Blood 2008 111: 975-976. [Full Text] [PDF] This article has been cited by other articles: C. E. Burns, J. L. Galloway, A. C. H. Smith, M. D. Keefe, T. J. Cashman, E. J. Paik, E. A. Mayhall, A. H. Amsterdam, and L. I. Zon A genetic screen in zebrafish defines a hierarchical network of pathways required for hematopoietic stem cell emergence Blood, June 4, 2009; 113(23): 5776 - 5782. [Abstract] [Full Text] [PDF] C. Hall, M. V. Flores, A. Chien, A. Davidson, K. Crosier, and P. 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