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Blood, 1 March 2008, Vol. 111, No. 5, pp. 2631-2635. Prepublished online as a Blood First Edition Paper on December 17, 2007; DOI 10.1182/blood-2007-07-099622. This Article Full Text (PDF) Supplemental Methods and Tables All Versions of this Article: blood-2007-07-099622v1 111/5/2631    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Delorme, B. Articles by Charbord, P. Search for Related Content PubMed PubMed Citation Articles by Delorme, B. Articles by Charbord, P. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 11, 2007 Accepted November 26, 2007 Specific plasma membrane protein phenotype of culture-amplified and native human bone marrow mesenchymal stem cells Bruno Delorme*, Jochen Ringe, Nathalie Gallay, Yves LeVern, Dominique Kerboeuf, Christian Jorgensen, Philippe Rosset, Luc Sensebe, Pierre Layrolle, Thomas Haupl, and Pierre Charbord INSERM, Equipe ESPRI/EA-3855, University Francois Rabelais, Faculty of Medicine, Tours, France Department of Rheumatology and Clinical Immunology, Laboratory for tissue engineering, Charite-University Medicine, Berlin, Germany Flow cytometry laboratory, UR IASP, INRA, Nouzilly, France INSERM, U844, Lapeyronie Hospital, Montpellier, France Department of Orthopedic Surgery, University Hospital, Tours, France Etablissement Francais du Sang-Centre-Atlantique, Tours, France INSERM, U791, Faculty of Dental Surgery, France, France * Corresponding author; email: delorme{at}med.univ-tours.fr. We have studied the plasma membrane protein phenotype of human culture-amplified and native Bone Marrow Mesenchymal Stem Cells (BM MSCs). We have found, using microarrays and flow cytometry, that cultured cells express specifically 113 transcripts and 17 proteins that were not detected in hematopoietic cells. These antigens define a lineage-homogenous cell population of mesenchymal cells, clearly distinct from the hematopoietic lineages, and distinguishable from other cultured skeletal mesenchymal cells (periosteal cells and synovial fibroblasts). Among the specific membrane proteins present on cultured MSCs, 9 allowed the isolation from BM mononuclear cells of a minute population of native MSCs. The enrichment in Colony-Forming Units-Fibroblasts was low for CD49b, CD90 and CD105, but high for CD73, CD130, CD146, CD200 and integrin alphaV/beta5. Additionally, the expression of CD73, CD146 and CD200 was down-regulated in differentiated cells. The new marker CD200, because of its specificity and immunomodulatory properties, deserves further in depth studies. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: B. Kutlu, A. G. Kayali, S. Jung, G. Parnaud, D. Baxter, G. Glusman, N. Goodman, L. A. Behie, A. Hayek, and L. Hood Meta-analysis of gene expression in human pancreatic islets after in vitro expansion Physiol Genomics, September 1, 2009; 39(1): 72 - 81. [Abstract] [Full Text] [PDF] C. Tripodo, A. Di Bernardo, M. P. Ternullo, C. Guarnotta, R. Porcasi, S. Ingrao, U. Gianelli, E. Boveri, E. Iannitto, G. Franco, et al. CD146+ bone marrow osteoprogenitors increase in the advanced stages of primary myelofibrosis Haematologica, January 1, 2009; 94(1): 127 - 130. [Abstract] [Full Text] [PDF] R. Summer and A. Fine Mesenchymal Progenitor Cell Research: Limitations and Recommendations Proceedings of the ATS, August 15, 2008; 5(6): 707 - 710. [Abstract] [Full Text] [PDF]

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