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 Blood, 1 March 2008, Vol. 111, No. 5, pp. 2631-2635.
Prepublished online as a Blood First Edition Paper on December 17, 2007; DOI 10.1182/blood-2007-07-099622.

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Submitted July 11, 2007
Accepted November 26, 2007

Specific plasma membrane protein phenotype of culture-amplified and native human bone marrow mesenchymal stem cells

Bruno Delorme*, Jochen Ringe, Nathalie Gallay, Yves LeVern, Dominique Kerboeuf, Christian Jorgensen, Philippe Rosset, Luc Sensebe, Pierre Layrolle, Thomas Haupl, and Pierre Charbord

INSERM, Equipe ESPRI/EA-3855, University Francois Rabelais, Faculty of Medicine, Tours, France
Department of Rheumatology and Clinical Immunology, Laboratory for tissue engineering, Charite-University Medicine, Berlin, Germany
Flow cytometry laboratory, UR IASP, INRA, Nouzilly, France
INSERM, U844, Lapeyronie Hospital, Montpellier, France
Department of Orthopedic Surgery, University Hospital, Tours, France
Etablissement Francais du Sang-Centre-Atlantique, Tours, France
INSERM, U791, Faculty of Dental Surgery, France, France

* Corresponding author; email: delorme{at}med.univ-tours.fr.

We have studied the plasma membrane protein phenotype of human culture-amplified and native Bone Marrow Mesenchymal Stem Cells (BM MSCs). We have found, using microarrays and flow cytometry, that cultured cells express specifically 113 transcripts and 17 proteins that were not detected in hematopoietic cells. These antigens define a lineage-homogenous cell population of mesenchymal cells, clearly distinct from the hematopoietic lineages, and distinguishable from other cultured skeletal mesenchymal cells (periosteal cells and synovial fibroblasts). Among the specific membrane proteins present on cultured MSCs, 9 allowed the isolation from BM mononuclear cells of a minute population of native MSCs. The enrichment in Colony-Forming Units-Fibroblasts was low for CD49b, CD90 and CD105, but high for CD73, CD130, CD146, CD200 and integrin alphaV/beta5. Additionally, the expression of CD73, CD146 and CD200 was down-regulated in differentiated cells. The new marker CD200, because of its specificity and immunomodulatory properties, deserves further in depth studies.


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