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Blood, 1 January 2008, Vol. 111, No. 1, pp. 42-49. Prepublished online as a Blood First Edition Paper on October 10, 2007; DOI 10.1182/blood-2007-07-099648. This Article Full Text (PDF) All Versions of this Article: blood-2007-07-099648v1 111/1/42    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wengner, A. M Articles by Rankin, S. M Search for Related Content PubMed PubMed Citation Articles by Wengner, A. M Articles by Rankin, S. M Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 6, 2007 Accepted September 17, 2007 The co-ordinated action of G-CSF and ELR+CXC chemokines in neutrophil mobilisation during acute inflammation Antje M Wengner, Simon C Pitchford, Rebecca C Furze, and Sara M Rankin* Leukocyte Biology Section, National Heart and Lung Institute, Faculty of Medicine, Imperial College, London, United Kingdom * Corresponding author; email: s.rankin{at}imperial.ac.uk. In this study we have identified a unique combinatorial effect of the chemokines KC/MIP-2 and the cytokine G-CSF with respect to the rapid mobilisation of neutrophils from the bone marrow in a model of acute peritonitis. 2h following an intraperitoneal injection of thioglycollate there was a 4.5 fold increase in blood neutrophil numbers which was inhibited by 84% and 72% prior administration of blocking mAbs against either the chemokines KC/ MIP-2 or G-CSF, respectively. An i.p. injection of G-CSF acted remotely to stimulate neutrophil mobilisation, but did not elicit recruitment into the peritoneum. Further, in vitro G -CSF was neither chemotactic nor chemokinetic for murine neutrophils and had no priming effect on chemotaxis stimulated by chemokines. Here we show that, in vitro and in vivo, G-CSF induces neutrophil mobilisation by disrupting their SDF-1alpha-mediated retention in the bone marrow. Using an in situ perfusion system of the mouse femoral bone marrow to directly assess mobilisation, KC and G-CSF mobilised 6.8 x 106 and 5.4 x 106 neutrophils respectively, while the infusion of KC and G-CSF together mobilised 19.5 x 106 neutrophils, indicating that these factors act co-operatively with respect to neutrophil mobilisation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. M. Soulika, E. Lee, E. McCauley, L. Miers, P. Bannerman, and D. Pleasure Initiation and Progression of Axonopathy in Experimental Autoimmune Encephalomyelitis J. Neurosci., November 25, 2009; 29(47): 14965 - 14979. [Abstract] [Full Text] [PDF] T. Loos, G. Opdenakker, J. Van Damme, and P. Proost Citrullination of CXCL8 increases this chemokine's ability to mobilize neutrophils into the blood circulation Haematologica, October 1, 2009; 94(10): 1346 - 1353. [Abstract] [Full Text] [PDF] J. L. Johns, K. C. MacNamara, N. J. Walker, G. M. Winslow, and D. L. Borjesson Infection with Anaplasma phagocytophilum Induces Multilineage Alterations in Hematopoietic Progenitor Cells and Peripheral Blood Cells Infect. Immun., September 1, 2009; 77(9): 4070 - 4080. [Abstract] [Full Text] [PDF] K. J. Eash, J. M. Means, D. W. White, and D. C. Link CXCR4 is a key regulator of neutrophil release from the bone marrow under basal and stress granulopoiesis conditions Blood, May 7, 2009; 113(19): 4711 - 4719. [Abstract] [Full Text] [PDF] J. M. Petty, C. C. Lenox, D. J. Weiss, M. E. Poynter, and B. T. Suratt Crosstalk between CXCR4/Stromal Derived Factor-1 and VLA-4/VCAM-1 Pathways Regulates Neutrophil Retention in the Bone Marrow J. Immunol., January 1, 2009; 182(1): 604 - 612. [Abstract] [Full Text] [PDF] A. P. Martin, S. Rankin, S. Pitchford, I. F. Charo, G. C. Furtado, and S. A. Lira Increased Expression of CCL2 in Insulin-Producing Cells of Transgenic Mice Promotes Mobilization of Myeloid Cells From the Bone Marrow, Marked Insulitis, and Diabetes Diabetes, November 1, 2008; 57(11): 3025 - 3033. [Abstract] [Full Text] [PDF] Y. Sawanobori, S. Ueha, M. Kurachi, T. Shimaoka, J. E. Talmadge, J. Abe, Y. Shono, M. Kitabatake, K. Kakimi, N. Mukaida, et al. Chemokine-mediated rapid turnover of myeloid-derived suppressor cells in tumor-bearing mice Blood, June 15, 2008; 111(12): 5457 - 5466. [Abstract] [Full Text] [PDF]

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