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Blood, 15 January 2008, Vol. 111, No. 2, pp. 806-815. Prepublished online as a Blood First Edition Paper on October 12, 2007; DOI 10.1182/blood-2007-07-101139. This Article Full Text (PDF) All Versions of this Article: blood-2007-07-101139v1 111/2/806    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Fagerli, U.-M. Articles by Borset, M. Search for Related Content PubMed PubMed Citation Articles by Fagerli, U.-M. Articles by Borset, M. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 12, 2007 Accepted September 30, 2007 Overexpression and involvement in migration by the metastasis-associated phosphatase PRL-3 in human myeloma cells Unn-Merete Fagerli*, Randi Utne Holt, Toril Holien, Thea Kristin Vaatsveen, Fenghuang Zhan, Kjartan W Egeberg, Bart Barlogie, Anders Waage, Harald Aarset, Hong Yan Dai, John D. Shaughnessy Jr, Anders Sundan, and Magne Borset Department of Oncology, St. Olavs University Hospital, Trondheim, Norway Faculty of Technology, Sor-Trondelag University College, Trondheim, Norway Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway Myeloma Institute for Research and Therapy, University Arkansas for Medical Sciences (UAMS), Little Rock, AR, United States Department of Hematology, St. Olavs University Hospital, Trondheim, Norway Department of Pathology and Molecular Genetics, St. Olavs University Hospital, Trondheim, Norway Department of Immunology, St. Olavs University Hospital, Trondheim, Norway * Corresponding author; email: unn-merete.fagerli{at}ntnu.no. Multiple myeloma (MM) is characterized by accumulation and dissemination of malignant plasma cells (PCs) in the bone marrow (BM). Gene expression profiling of two MM cell lines (OH-2 and IH-1) indicated that expression of PRL-3, a metastasis-associated tyrosine phosphatase, was induced by several mitogenic cytokines. Cytokine-driven PRL-3 expression could be shown in several myeloma cell lines both at the mRNA and protein level. There was significantly higher expression of the PRL-3 gene in PCs from patients with Monoclonal Gammopathy of Undetermined Significance (MGUS), Smouldering myeloma (SMM) and myeloma than in normal PCs. Among 7 MM subgroups identified by unsupervised hierarchical cluster analysis, PRL-3 gene expression was significant higher in the 3 groups denoted as Proliferation, Low Bone Disease, and MMSET/FGFR3. PRL-3 protein was detected in 18 out of 20 BM biopsies from patients with MM. Silencing of the PRL-3 gene by siRNA reduced cell migration in the MM cell line INA-6, but had no detectable effect on proliferation and cell cycle phase distribution of the cells. In conclusion, PRL-3 is a gene product specifically expressed in malignant plasma cells and may have a role in migration of these cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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