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Blood, 15 April 2008, Vol. 111, No. 8, pp. 4220-4232. Prepublished online as a Blood First Edition Paper on November 26, 2007; DOI 10.1182/blood-2007-07-101691. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2007-07-101691v1 111/8/4220    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chen, Y. Articles by Chen, Z. W. Search for Related Content PubMed PubMed Citation Articles by Chen, Y. Articles by Chen, Z. W. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 18, 2007 Accepted November 8, 2007 NSOM/QD-based nanoscale immune-fluorescence imaging of antigen-specific T cell receptor responses during an in vivo clonal V2V2 T cell expansion Yong Chen, Lingyun Shao, Zahida Ali, Jiye Cai, and Zheng W. Chen* Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, IL, United States Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China Department of Chemistry, Jinan University, Guangzhou, China * Corresponding author; email: zchen{at}uic.edu. Nanoscale imaging of an in vivo antigen-specific T cell immune response has not been reported. Here, the combined NSOM- and fluorescent QD-based nanotechnology was employed to perform immune-fluorescence imaging of antigen-specific TCR response in an in vivo model of clonal T cell expansion. The NSOM/QD system provided a best-optical-resolution nanoscale imaging of V2V2 TCR on the membrane of non-stimulated V2V2 T cells. Prior to Ag-induced clonal expansion, these non-stimulating V2V2 TCR appeared to be distributed differently from their TCR counterparts on cell-surface. Surprisingly, V2V2 TCR nanoclusters not only were formed but also sustained on the membrane during an in vivo clonal expansion of V2V2 T cells after phosphoantigen treatment. The TCR nanoclusters could array to form nanodomains or microdomains on the membrane of clonally-expanded V2V2 T cells. Interestingly, expanded V2V2 T cells bearing TCR nanoclusters or nanodomains were able to recognize phosphoantigen and to exert better effector function. These studies provided nanoscale insight into the in vivo T cell immune response. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: Y. Wu, W. Wu, W. M. Wong, E. Ward, A. J. Thrasher, D. Goldblatt, M. Osman, P. Digard, D. H. Canaday, and K. Gustafsson Human {gamma}{delta} T Cells: A Lymphoid Lineage Cell Capable of Professional Phagocytosis J. Immunol., November 1, 2009; 183(9): 5622 - 5629. [Abstract] [Full Text] [PDF] Y. Chen, J. Qin, and Z. W. Chen Fluorescence-topographic NSOM directly visualizes peak-valley polarities of GM1/GM3 rafts in cell membrane fluctuations J. Lipid Res., October 1, 2008; 49(10): 2268 - 2275. [Abstract] [Full Text] [PDF] H. Wei, D. Huang, X. Lai, M. Chen, W. Zhong, R. Wang, and Z. W. Chen Definition of APC Presentation of Phosphoantigen (E)-4-Hydroxy-3-methyl-but-2-enyl Pyrophosphate to V{gamma}2V{delta}2 TCR J. Immunol., October 1, 2008; 181(7): 4798 - 4806. [Abstract] [Full Text] [PDF]

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