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Blood, 1 February 2008, Vol. 111, No. 3, pp. 1489-1496. Prepublished online as a Blood First Edition Paper on November 21, 2007; DOI 10.1182/blood-2007-07-102111. This Article Full Text (PDF) Supplemental Video All Versions of this Article: blood-2007-07-102111v1 111/3/1489    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kambayashi, T. Articles by Koretzky, G. A Search for Related Content PubMed PubMed Citation Articles by Kambayashi, T. Articles by Koretzky, G. A Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 19, 2007 Accepted November 13, 2007 Indirect involvement of allergen-captured mast cells in antigen presentation Taku Kambayashi, Jan D Baranski, Rebecca G Baker, Tao Zou, Eric J Allenspach, Jonathan E Shoag, Peter L Jones, and Gary A Koretzky* Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, PA, United States Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, United States Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, United States Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA, United States Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, United States * Corresponding author; email: koretzky{at}mail.med.upenn.edu. It is generally believed that mast cells influence T cell activation non-specifically through the release of inflammatory mediators. In this report, we provide evidence that mast cells may also affect antigen-specific T cell responses by internalizing IgE-bound antigens for presentation to antigen-specific T cells. Surprisingly, T cell activation did not require that mast cells express MHC class II, indicating that mast cells were not involved in the direct presentation of the internalized antigens. Rather, the antigen captured by mast cells is presented by other MHC class II+ antigen-presenting cells. To explore how this may occur, we investigated the fate of mast cells stimulated by antigen and found that FcRI crosslinking enhances mast cell apoptosis. Cell death by antigen-captured mast cells was required for efficient presentation, since protection of mast cell death significantly decreased T cell activation. These results suggest that mast cells may be involved in antigen presentation by acting as an antigen reservoir after antigen capture through specific IgE molecules bound to their FcRI. This mechanism may contribute to the positive effect of mast cells on the development of T cell responses. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Mast cells and T-cell expansion Susumu Nakae, Keisuke Oboki, and Hirohisa Saito Blood 2008 111: 2497-2498. [Full Text] [PDF] This article has been cited by other articles: T. Kambayashi, E. J. Allenspach, J. T. Chang, T. Zou, J. E. Shoag, S. L. Reiner, A. J. Caton, and G. A. Koretzky Inducible MHC Class II Expression by Mast Cells Supports Effector and Regulatory T Cell Activation J. Immunol., April 15, 2009; 182(8): 4686 - 4695. [Abstract] [Full Text] [PDF]

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