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Blood, 1 January 2008, Vol. 111, No. 1, pp. 142-149. Prepublished online as a Blood First Edition Paper on September 28, 2007; DOI 10.1182/blood-2007-07-102558. This Article Full Text (PDF) Supplemental Figure All Versions of this Article: blood-2007-07-102558v1 111/1/142    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jeannet, G. Articles by Held, W. Search for Related Content PubMed PubMed Citation Articles by Jeannet, G. Articles by Held, W. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 25, 2007 Accepted September 24, 2007 Long-term, multilineage hematopoiesis occurs in the combined absence of -catenin and -catenin Gregoire Jeannet, Marina Scheller, Leonardo Scarpellino, Stephane Duboux, Noemie Gardiol, Jonathan Back, Fabien Kuttler, Ilaria Malanchi, Walter Birchmeier, Achim Leutz, Joerg Huelsken, and Werner Held* Lausanne Branch, Ludwig Institute for Cancer Research, Epalinges, Switzerland Max-Delbruck Center for Molecular Medicine, Berlin, Germany Swiss Institute for Experimental Cancer Research, Ecole Polytechnique Federale de Lausanne, Epalinges, Switzerland * Corresponding author; email: werner.held{at}isrec.unil.ch. The canonical Wnt signaling pathway plays key roles in stem cell maintenance, progenitor cell expansion and lineage decisions. Transcriptional responses induced by Wnt depend on the association of either -catenin or -catenin with TCF/LEF transcription factors. Here we show that hematopoiesis, including thymopoiesis, is normal in the combined absence of - and -catenin. Double-deficient hematopoietic stem cells (HSCs) maintain long-term repopulation capacity and multi lineage differentiation potential. Unexpectedly, two independent ex vivo reporter gene assays show that Wnt signal transmission is maintained in double-deficient HSCs, thymocytes, or peripheral T cells. In contrast, Wnt signaling is strongly reduced in thymocytes lacking TCF-1 or in non-hematopoietic cells devoid of -catenin. These data provide the first evidence that hematopoietic cells can transduce canonical Wnt signals in the combined absence of - and -catenin. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. Kovalovsky, Y. Yu, M. Dose, A. Emmanouilidou, T. Konstantinou, K. Germar, K. Aghajani, Z. Guo, M. Mandal, and F. Gounari {beta}-Catenin/Tcf Determines the Outcome of Thymic Selection in Response to {alpha}{beta}TCR Signaling J. Immunol., September 15, 2009; 183(6): 3873 - 3884. [Abstract] [Full Text] [PDF] M. Xu, A. Sharma, D. L. Wiest, and J. M. Sen Pre-TCR-Induced {beta}-Catenin Facilitates Traversal through {beta}-Selection J. Immunol., January 15, 2009; 182(2): 751 - 758. [Abstract] [Full Text] [PDF] T. C. Luis, F. Weerkamp, B. A. E. Naber, M. R. M. Baert, E. F. E. de Haas, T. Nikolic, S. Heuvelmans, R. R. De Krijger, J. J. M. van Dongen, and F. J. T. Staal Wnt3a deficiency irreversibly impairs hematopoietic stem cell self-renewal and leads to defects in progenitor cell differentiation Blood, January 15, 2009; 113(3): 546 - 554. [Abstract] [Full Text] [PDF] S. Malhotra, Y. Baba, K. P. Garrett, F. J. T. Staal, R. Gerstein, and P. W. Kincade Contrasting Responses of Lymphoid Progenitors to Canonical and Noncanonical Wnt Signals J. Immunol., September 15, 2008; 181(6): 3955 - 3964. [Abstract] [Full Text] [PDF] T. Grigoryan, P. Wend, A. Klaus, and W. Birchmeier Deciphering the function of canonical Wnt signals in development and disease: conditional loss- and gain-of-function mutations of {beta}-catenin in mice Genes & Dev., September 1, 2008; 22(17): 2308 - 2341. [Abstract] [Full Text] [PDF]

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