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Blood, 15 February 2008, Vol. 111, No. 4, pp. 1789-1796. Prepublished online as a Blood First Edition Paper on November 27, 2007; DOI 10.1182/blood-2007-07-104364. This Article Full Text (PDF) All Versions of this Article: blood-2007-07-104364v1 111/4/1789    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Vonakis, B. M. Articles by MacDonald, S. M. Search for Related Content PubMed PubMed Citation Articles by Vonakis, B. M. Articles by MacDonald, S. M. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 31, 2007 Accepted November 15, 2007 Distinct characteristics of signal transduction events by histamine releasing factor/translationally controlled tumor protein (HRF/TCTP)-induced priming and activation of human basophils Becky M. Vonakis, Donald W MacGlashan Jr., Natalia Vilarino, Jacqueline M Langdon, Rebecca S. Scott, and Susan M. MacDonald* Division of Allergy and Clinical Immunolgy, The Johns Hopkins Asthma and Allergy Center, Johns Hopkins University, Baltimore, MD, United States * Corresponding author; email: smacdona{at}jhmi.edu. We previously identified a negative correlation between histamine release to Histamine Releasing Factor/Translationally Controlled Tumor Protein (HRF/TCTP) and protein levels of the Src homology 2 domain-containing inositol 5' phosphatase (SHIP) in basophils. We have also demonstrated that HRF/TCTP primes basophils to release mediators. The purpose of this study was to begin characterization of signal transduction events directly induced by HRF/TCTP and to investigate these events when HRF/TCTP is used as a priming agent for human basophil histamine release. Highly purified human basophils were examined for surface expression of bound HRF/TCTP, changes in calcium, phosphorylation of Akt, MEK, ERK, Syk and FcRI. Results showed that basophils from all donors bound HRF/TCTP. There was a biphasic calcium response to HRF/TCTP, which corresponded to the magnitude of histamine release. Furthermore, those donors who have direct histamine release when exposed to HRF/TCTP (HRF/TCTP-responders [HRF/TCTP-R]) have phosphorylation of Syk, Akt, MEK and ERK. Remarkably, basophils from HRF/TCTP-nonresponder donors HRF/TCTP-NR) do not show phosphorylation of these molecules. This finding is different from IL-3, that also primes basophils for histamine release, but does show phosphorylation of these events. We conclude that priming induced by HRF/TCTP is distinct from that induced by IL-3. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. M. Langdon, J. T. Schroeder, B. M. Vonakis, A. P. Bieneman, K. Chichester, and S. M. MacDonald Histamine-releasing factor/translationally controlled tumor protein (HRF/TCTP)-induced histamine release is enhanced with SHIP-1 knockdown in cultured human mast cell and basophil models J. Leukoc. Biol., October 1, 2008; 84(4): 1151 - 1158. [Abstract] [Full Text] [PDF]

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