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Blood, 1 April 2008, Vol. 111, No. 7, pp. 3507-3513. Prepublished online as a Blood First Edition Paper on January 22, 2008January 18, 2008; DOI 10.1182/blood-2007-08-106229. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2007-08-106229v1 blood-2007-08-106229v2 111/7/3507    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Okorie, U. Articles by Diamond, S. L. Search for Related Content PubMed PubMed Citation Articles by Okorie, U. Articles by Diamond, S. L. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted August 10, 2007 Accepted January 4, 2008 Determination of surface tissue factor thresholds that trigger coagulation at venous and arterial shear rates: amplification of 100 fM circulating tissue factor requires flow Uzoma Okorie, William S. Denney, Manash S Chatterjee, Keith B Neeves, and Scott L. Diamond* Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA, United States * Corresponding author; email: sld{at}seas.upenn.edu. Protein microarrays presenting spots of collagen and lipidated tissue factor (TF) allowed a determination of the critical surface concentration of TF required to trigger coagulation under flow. Whole blood supplemented with corn trypsin inhibitor (to inhibit Factor XIIa) was perfused over microarrays for 5 min. Immunofluorescence staining of platelet glycoprotein GPIb and fibrin(ogen) revealed a critical TF concentration (EC50) of 3.6, 8.4, and 10.2 molecules-TF/µm2 at wall shear rates of 100, 500, and 1000 s-1, respectively. For collagen arrays where only the center lane of spots (in the direction of flow) contained TF, a downstream distance of 14 mm was required for the thrombus to widen enough to reach across a 300 micron gap to the adjacent TF-free lanes of collagen spots, in agreement with numerical simulation. To investigate the effect of low levels of circulating TF, whole blood (± 100 fM of added TF) was tested under static and flow conditions. After 5 min, the addition of 100 fM TF to whole blood had negligible effect under static conditions, but caused a 2.5-fold increase in fibrin formation under flow. This report defines the threshold concentrations of surface TF required to trigger coagulation under flow. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. Butenas, T. Orfeo, and K. G. Mann Tissue Factor in Coagulation: Which? Where? When? Arterioscler Thromb Vasc Biol, December 1, 2009; 29(12): 1989 - 1996. [Abstract] [Full Text] [PDF] R. A. Campbell, K. A. Overmyer, C. H. Selzman, B. C. Sheridan, and A. S. Wolberg Contributions of extravascular and intravascular cells to fibrin network formation, structure, and stability Blood, November 26, 2009; 114(23): 4886 - 4896. [Abstract] [Full Text] [PDF] F. Shen, C. J. Kastrup, Y. Liu, and R. F. Ismagilov Threshold Response of Initiation of Blood Coagulation by Tissue Factor in Patterned Microfluidic Capillaries Is Controlled by Shear Rate Arterioscler Thromb Vasc Biol, November 1, 2008; 28(11): 2035 - 2041. [Abstract] [Full Text] [PDF]

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