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Blood, 1 April 2008, Vol. 111, No. 7, pp. 3435-3438. Prepublished online as a Blood First Edition Paper on October 11, 2007; DOI 10.1182/blood-2007-08-107086. This Article Full Text (PDF) Supplemental Table All Versions of this Article: blood-2007-08-107086v1 111/7/3435    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lux, C. T. Articles by Yoder, M. C. Search for Related Content PubMed PubMed Citation Articles by Lux, C. T. Articles by Yoder, M. C. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted August 14, 2007 Accepted September 28, 2007 All primitive and definitive hematopoietic progenitor cells emerging prior to E10 in the mouse embryo are products of the yolk sac Christopher Thomas Lux, Momoko Yoshimoto, Kathleen McGrath, Simon J. Conway, James Palis, and Mervin C. Yoder* Dept. of Pediatrics, Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN, United States Dept. of Pediatrics, Center for Pediatric Biomedical Research, University of Rochester School of Medicine, Rochester, NY, United States * Corresponding author; email: myoder{at}iupui.edu. The relative contribution of yolk sac (YS) derived cells to the circulating definitive hematopoietic progenitor cell (HPC) pool that seeds the fetal liver remains controversial due to the presence of systemic circulation and the onset of hematopoiesis within the embryo proper (EP) prior to liver seeding. Ncx1-/- embryos fail to initiate a heartbeat on embryonic day (E) 8.25, but continue to develop through E10. We detected normal numbers of primitive erythroid progenitors in Ncx1-/- versus wild type (WT) YS, but primitive erythroblasts did not circulate in the Ncx1-/- EP. While there was no significant difference in the number of definitive HPCs in Ncx1-/- versus WT YS through E9.5, the Ncx1-/- EP was nearly devoid of HPCs. Thus, primitive erythroblasts and essentially all definitive HPC destined to initially seed the fetal liver after E9.5 are generated in the YS between E7.0-E9.5 and are redistributed into the EP via the systemic circulation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: AGM: maternity ward or finishing school? Donald Metcalf Blood 2008 111: 3305-3306. [Full Text] [PDF] This article has been cited by other articles: D. J. Stumpo, H. E. Broxmeyer, T. Ward, S. Cooper, G. Hangoc, Y. J. Chung, W. C. Shelley, E. K. Richfield, M. K. Ray, M. C. Yoder, et al. Targeted disruption of Zfp36l2, encoding a CCCH tandem zinc finger RNA-binding protein, results in defective hematopoiesis Blood, September 17, 2009; 114(12): 2401 - 2410. [Abstract] [Full Text] [PDF] E. Yi Ni Lam, J. Y. M. Chau, M. L. Kalev-Zylinska, T. M. Fountaine, R. S. Mead, C. J. Hall, P. S. Crosier, K. E. Crosier, and M. Vega Flores Zebrafish runx1 promoter-EGFP transgenics mark discrete sites of definitive blood progenitors Blood, February 5, 2009; 113(6): 1241 - 1249. [Abstract] [Full Text] [PDF] E. Dzierzak and A. Medvinsky The discovery of a source of adult hematopoietic cells in the embryo Development, July 15, 2008; 135(14): 2343 - 2346. [Abstract] [Full Text] [PDF] N. C. Dubois, C. Adolphe, A. Ehninger, R. A. Wang, E. J. Robertson, and A. Trumpp Placental rescue reveals a sole requirement for c-Myc in embryonic erythroblast survival and hematopoietic stem cell function Development, July 15, 2008; 135(14): 2455 - 2465. [Abstract] [Full Text] [PDF] J. Tober, K. E. McGrath, and J. Palis Primitive erythropoiesis and megakaryopoiesis in the yolk sac are independent of c-myb Blood, March 1, 2008; 111(5): 2636 - 2639. [Abstract] [Full Text] [PDF]

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