SEARCH:   Advanced

Blood, 1 February 2008, Vol. 111, No. 3, pp. 1208-1216. Prepublished online as a Blood First Edition Paper on October 18, 2007; DOI 10.1182/blood-2007-08-107698. This Article Full Text (PDF) Supplemental Methods and Figures All Versions of this Article: blood-2007-08-107698v1 111/3/1208    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Egorina, E. M. Articles by Osterud, B. Search for Related Content PubMed PubMed Citation Articles by Egorina, E. M. Articles by Osterud, B. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted August 16, 2007 Accepted October 8, 2007 Granulocytes do not express but acquire monocyte-derived tissue factor in whole blood: evidence for a direct transfer Elena M. Egorina*, Mikhail A. Sovershaev, Jan O. Olsen, and Bjarne Osterud Department of Biochemistry, Institute of Medical Biology, University of Tromso, Tromso, Norway * Corresponding author; email: egorina{at}fagmed.uit.no. Unlike unanimous opinion on tissue factor (TF) expression in monocytes, the quest for TF presence in granulocytes has been going on for decades. In order to study the cell origin and track the blood-borne TF, we assessed TF activity and protein levels, knocked-down endogenous TF expression with small interfering RNA (siRNA), and overexpressed TF-yellow fluorescent protein (TF-YFP) fusion in immunologically isolated human monocytes and granulocytes. Monocytes and, to a much lesser extent, granulocytes isolated from lipopolysaccharide (LPS)/phorbol 12-myristate-13-acetate (PMA)-stimulated whole blood contained active TF antigen. However, only monocytes possessed significant TF activity and protein levels when stimulated with LPS/PMA in suspension. Reintroduction of TF-silenced monocytes to whole blood led to a profound reduction of LPS/PMA-stimulated TF activity in both mononuclear cell (MNC) and granulocyte fractions. No reduction in TF activity in MNC and granulocyte fractions was observed when TF-silenced granulocytes were reintroduced to whole blood. As shown by immunoblotting, flow cytometry and confocal microscopy, granulocytes became positive for TF-YFP when isolated from whole blood reconstituted with TF-YFP-expressing monocytes. Taken together, we pinpoint monocytes as a major source of TF and provide solid experimental evidence for a direct transfer of TF protein from the monocytes to granulocytes in the blood. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Tissue factor in granulocytes: yes and NO L. Vijaya Mohan Rao Blood 2008 111: 976-977. [Full Text] [PDF] This article has been cited by other articles: M. A. Sovershaev, E. M. Egorina, J.-B. Hansen, B. Osterud, P. Pacher, J.-P. Stasch, and O. V. Evgenov Soluble Guanylate Cyclase Agonists Inhibit Expression and Procoagulant Activity of Tissue Factor Arterioscler Thromb Vasc Biol, October 1, 2009; 29(10): 1578 - 1586. [Abstract] [Full Text] [PDF]

	Copyright © 2007 by American Society of Hematology         Online ISSN: 1528-0020