Submitted September 19, 2007
Accepted November 19, 2007
Lipopolysaccharide binding protein promoter variants influence the risk for Gram-negative bacteremia and mortality after allogeneic hematopoietic cell transplantation
Jason W. Chien*, Michael J. Boeckh, John A. Hansen, and Joan G. Clark
Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, United States
* Corresponding author; email: jchien{at}fhcrc.org.
Lipopolysaccharide binding protein (LBP) function is dependent upon circulating LBP levels. Disturbance of LBP transcription regulation may influence the risk for clinical events. In a nested case control study using a single nucleotide polymorphism haplotype tagging (tagSNP) approach, we assessed whether genetic variation in the LBP gene influences the risk for GN bacteremia after allogeneic hematopoietic cell transplantation (HCT), then validated the association in a prospective cohort by correlating genetic variation with basal serum LBP levels and mortality. Presence of the tagSNP 6878 C allele among patients was associated with a two-fold higher risk for GN bacteremia (odds ratio 2.15, 95% confidence interval [CI] 1.31-3.52, p=0.002). TagSNP 6878 was in strong linkage disequilibrium with three SNPs in the LBP promoter, one of which was SNP 1683 (r2=0.8), located in a CAAT box that regulates LBP promoter efficiency. SNP 1683 was associated with higher median basal serum LBP levels (TT 8.07 µg/ml; TC 10.40 µg/ml; CC 17.39 µg/ml; p=0.002), and a 5-fold increase in GN bacteremia related mortality after HCT (hazard ratio [HR] 4.83, 95% CI 1.38-16.75, p=0.013). These data suggest that transcriptional regulation of the LBP gene contributes to the risk for developing GN bacteremia and death after HCT.
