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Blood, 1 May 2008, Vol. 111, No. 9, pp. 4500-4510. Prepublished online as a Blood First Edition Paper on February 12, 2008; DOI 10.1182/blood-2007-09-110569. This Article Full Text (PDF) Supplemental Table and Figures All Versions of this Article: blood-2007-09-110569v1 111/9/4500    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sumanas, S. Articles by Lin, S. Search for Related Content PubMed PubMed Citation Articles by Sumanas, S. Articles by Lin, S. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 6, 2007 Accepted February 4, 2008 Interplay between Etsrp/ER71, scl and alk8 signaling controls endothelial and myeloid cell formation Saulius Sumanas, Gustavo Gomez, Yan Zhao, Changwon Park, Kyunghee Choi, and Shuo Lin* Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, United States Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, United States Molecular Cell Biology Program, Washington University School of Medicine, St. Louis, MO, United States * Corresponding author; email: shuolin{at}ucla.edu. Vascular endothelial and myeloid cells have been proposed to originate from a common precursor cell, hemangioblast. Mechanism of endothelial and myeloid cell specification and differentiation is poorly understood. We have previously described the endothelial-specific zebrafish Ets1-related protein (Etsrp), which was both necessary and sufficient to initiate vasculogenesis in the zebrafish embryos. Here we identify human Etv2/ER71 and mouse ER71 proteins as functional orthologs of Etsrp. Overexpression of mouse ER71 and Etsrp caused strong expansion of hemangioblast and vascular endothelial lineages in a zebrafish embryo. In addition, we show that etsrp is also required for the formation of myeloid but not erythroid cells. In the absence of etsrp function, the number of granulocytes and macrophages is greatly reduced. Etsrp overexpression causes expansion of both myeloid and vascular endothelial lineages. Analysis of mosaic embryos indicates that etsrp functions cell-autonomously in inducing myeloid lineage. We further demonstrate that the choice of endothelial versus myeloid fate depends on combinatorial effect of etsrp, scl and alk8 genes. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. Peterkin, A. Gibson, and R. Patient Common genetic control of haemangioblast and cardiac development in zebrafish Development, May 1, 2009; 136(9): 1465 - 1474. [Abstract] [Full Text] [PDF] A. Bukrinsky, K. J. P. Griffin, Y. Zhao, S. Lin, and U. Banerjee Essential role of spi-1-like (spi-1l) in zebrafish myeloid cell differentiation Blood, February 26, 2009; 113(9): 2038 - 2046. [Abstract] [Full Text] [PDF] A. Ferdous, A. Caprioli, M. Iacovino, C. M. Martin, J. Morris, J. A. Richardson, S. Latif, R. E. Hammer, R. P. Harvey, E. N. Olson, et al. Nkx2-5 transactivates the Ets-related protein 71 gene and specifies an endothelial/endocardial fate in the developing embryo PNAS, January 20, 2009; 106(3): 814 - 819. [Abstract] [Full Text] [PDF] F. Liu and R. Patient Genome-Wide Analysis of the Zebrafish ETS Family Identifies Three Genes Required for Hemangioblast Differentiation or Angiogenesis Circ. Res., November 7, 2008; 103(10): 1147 - 1154. [Abstract] [Full Text] [PDF]

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