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Blood, 1 February 2008, Vol. 111, No. 3, pp. 1295-1298. Prepublished online as a Blood First Edition Paper on October 10, 2007; DOI 10.1182/blood-2007-09-110700. This Article Full Text (PDF) All Versions of this Article: blood-2007-09-110700v1 111/3/1295    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Shida, Y. Articles by Yoshioka, A. Search for Related Content PubMed PubMed Citation Articles by Shida, Y. Articles by Yoshioka, A. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 5, 2007 Accepted October 3, 2007 Functional imaging of shear-dependent activity of ADAMTS13 in regulating mural thrombus growth under whole blood flow conditions Yasuaki Shida, Kenji Nishio, Mitsuhiko Sugimoto*, Tomohiro Mizuno, Masaaki Hamada, Seiji Kato, Masanori Matsumoto, Kazuo Okuchi, Yoshihiro Fujimura, and Akira Yoshioka Department of Pediatrics, Nara Medical University, Kashihara, Nara, Japan Department of Emergency and Critical Care Medicine, Nara Medical University, Kashihara, Nara, Japan Department of Blood Transfusion, Nara Medical University, Kashihara, Nara, Japan * Corresponding author; email: sugi-ped{at}naramed-u.ac.jp. The metalloprotease ADAMTS13 is assumed to regulate the functional levels of von Willebrand factor (VWF) appropriate for normal hemostasis in vivo by reducing VWF multimer size, which directly represents the thrombogenic activity of this factor. Using an in vitro perfusion chamber system, we studied the mechanisms of ADAMTS13 action during platelet thrombus formation on a collagen surface under whole blood flow conditions. Inhibition studies with a function-blocking anti-ADAMTS13 antibody, combined with immunostaining of thrombi with an anti-VWF monoclonal antibody that specifically reflects the VWF-cleaving activity of ADAMTS13, provided visual evidence for a shear rate-dependent action of ADAMTS13 that limits thrombus growth directly at the site of the on-going thrombus generation process. Our results identify an exquisitely specific regulatory mechanism that prevents arterial occlusion under high shear rate conditions during mural thrombogenesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Shear elegance: regulation of thrombus growth by shear stress William E. Hobbs, II and José A. López Blood 2008 111: 972. [Full Text] [PDF] This article has been cited by other articles: Z. Li, M. A. Nardi, Y.-S. Li, W. Zhang, R. Pan, S. Dang, H. Yee, D. Quartermain, S. Jonas, and S. Karpatkin C-terminal ADAMTS-18 fragment induces oxidative platelet fragmentation, dissolves platelet aggregates, and protects against carotid artery occlusion and cerebral stroke Blood, June 11, 2009; 113(24): 6051 - 6060. [Abstract] [Full Text] [PDF] F. Banno, A. K. Chauhan, K. Kokame, J. Yang, S. Miyata, D. D. Wagner, and T. Miyata The distal carboxyl-terminal domains of ADAMTS13 are required for regulation of in vivo thrombus formation Blood, May 21, 2009; 113(21): 5323 - 5329. [Abstract] [Full Text] [PDF] J. Rivera, M. L. Lozano, L. Navarro-Nunez, and V. Vicente Platelet receptors and signaling in the dynamics of thrombus formation Haematologica, May 1, 2009; 94(5): 700 - 711. [Abstract] [Full Text] [PDF]

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