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Blood, 1 July 2008, Vol. 112, No. 1, pp. 120-130. Prepublished online as a Blood First Edition Paper on March 6, 2008; DOI 10.1182/blood-2007-09-114181. This Article Full Text (PDF) All Versions of this Article: blood-2007-09-114181v1 112/1/120    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Liu, S. D. Articles by Miceli, M. C. Search for Related Content PubMed PubMed Citation Articles by Liu, S. D. Articles by Miceli, M. C. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 24, 2007 Accepted March 4, 2008 Endogenous galectin-1 enforces class I-restricted TCR functional fate decisions in thymocytes Scot D. Liu, Chan C Whiting, Tamar Tomassian, Mabel Pang, Stephanie J Bissel, Linda G Baum, Valeri V. Mossine, Francoise Poirier, and M. Carrie Miceli* Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, United States Ingenuity Systems, Partner & Professional Services, Redwood City, CA, United States Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, United States Department of Pathology and Laboratory Medicine, University of California, Los Angeles, Los Angeles, CA, United States Department of Biochemistry, University of Missouri, Columbia, MO, United States Institut Jacques Monod, Centre National de la Recherche Scientifique Unite Mixte de Recherche, Paris, France * Corresponding author; email: cmiceli{at}ucla.edu. During thymocyte development, the TCR can discriminate MHC/peptide ligands over a narrow range of affinities and translate subtle differences into functional fate decisions. How small differences in TCR input are translated into absolute differences in functional output is unclear. We examined the effects of galectin-1 ablation in the context of class-I-restricted thymocyte development. Galectin-1 expression opposed TCR partial agonist-driven positive selection, but promoted TCR agonist-driven negative selection of conventional CD8+ T cells. Galectin-1 expression also promoted TCR agonist-driven CD8 IEL development. Recombinant galectin-1 enhanced TCR binding to agonist/MHC complexes and promoted a negative-selection-signaling signature, reflected in intensified rapid and transient ERK activation. In contrast, galectin-1 expression antagonized ERK activity in thymocytes undergoing positive selection. We propose that galectin-1 aids in discriminating TCR-directed fate decisions by promoting TCR binding to agonist/MHC complexes and enforcing agonist-driven signals, while opposing partial-agonist signals. In this way, galectin-1 widens the distinction between TCR-directed functional fate cues. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Thymocyte-fate decisions: bittersweet new flavor Guy Werlen Blood 2008 112: 7-8. [Full Text] [PDF] This article has been cited by other articles: M. V. Tribulatti, V. Cattaneo, U. Hellman, J. Mucci, and O. Campetella Galectin-8 provides costimulatory and proliferative signals to T lymphocytes J. Leukoc. Biol., August 1, 2009; 86(2): 371 - 380. [Abstract] [Full Text] [PDF] M. Pang, J. He, P. Johnson, and L. G. Baum CD45-Mediated Fodrin Cleavage during Galectin-1 T Cell Death Promotes Phagocytic Clearance of Dying Cells J. Immunol., June 1, 2009; 182(11): 7001 - 7008. [Abstract] [Full Text] [PDF] A. Norambuena, C. Metz, L. Vicuna, A. Silva, E. Pardo, C. Oyanadel, L. Massardo, A. Gonzalez, and A. Soza Galectin-8 Induces Apoptosis in Jurkat T Cells by Phosphatidic Acid-mediated ERK1/2 Activation Supported by Protein Kinase A Down-regulation J. Biol. Chem., May 8, 2009; 284(19): 12670 - 12679. [Abstract] [Full Text] [PDF] L. V Norling, M. Perretti, and D. Cooper Endogenous galectins and the control of the host inflammatory response J. Endocrinol., May 1, 2009; 201(2): 169 - 184. [Abstract] [Full Text] [PDF] S. D. Liu, T. Tomassian, K. W. Bruhn, J. F. Miller, F. Poirier, and M. C. Miceli Galectin-1 Tunes TCR Binding and Signal Transduction to Regulate CD8 Burst Size J. Immunol., May 1, 2009; 182(9): 5283 - 5295. [Abstract] [Full Text] [PDF]

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