Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Future Articles
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
 Blood, 15 February 2008, Vol. 111, No. 4, pp. 2444-2451.
Prepublished online as a Blood First Edition Paper on November 30, 2007; DOI 10.1182/blood-2007-09-115006.

This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Figures
Right arrow All Versions of this Article:
blood-2007-09-115006v1
111/4/2444    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Weksberg, D. C
Right arrow Articles by Goodell, M. A
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Weksberg, D. C
Right arrow Articles by Goodell, M. A
Related Collections
Right arrowRelated Articles in Blood Online
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Next Article next article arrow

Submitted September 28, 2007
Accepted November 25, 2007

CD150 negative Side Population cells represent a functionally distinct population of long-term hematopoietic stem cells

David C Weksberg, Stuart M Chambers, Nathan C Boles, and Margaret A Goodell*

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, United States
Interdepartmental Program in Cell and Molecular Biology, Baylor College of Medicine, Houston, TX, United States
Stem Cells and Regenerative Medicine Center, Baylor College of Medicine, Houston, TX, United States

* Corresponding author; email: goodell{at}bcm.edu.

Hematopoietic stem cells are a self-renewing population of bone marrow cells which replenish the cellular elements of blood throughout life. HSCs represent a paradigm for the study of stem cell biology, as robust methods for prospective isolation of HSCs have facilitated rigorous characterization of these cells. Recently, a new isolation method was reported, using the SLAM family of cell surface markers, including CD150 (SlamF1), to offer potential advantages over established protocols. We examined the overlap between SLAM family member expression with an established isolation scheme based on Hoechst dye efflux (side population, SP) in conjunction with canonical HSC cell surface markers (Sca-1, c-Kit, and lineage markers). Importantly, we find that stringent gating of SLAM markers is essential to achieving purity in HSC isolation, and that the inclusion of canonical HSC markers in the SLAM scheme can greatly augment HSC purity. Furthermore, we observe that both CD150+ and CD150-cells can be found within the SP population, and that both populations can contribute to long-term multilineage reconstitution. Thus, using SLAM family markers to isolate HSCs neglects a substantial fraction of the marrow HSC compartment. Interestingly, these two subpopulations are functionally distinct, with respect to lineage output as well as proliferative status.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Articles in Blood Online:

CD150– cells are transiently reconstituting multipotent progenitors with little or no stem cell activity
Mark J. Kiel, Omer H. Yilmaz, and Sean J. Morrison
Blood 2008 111: 4413-4414. [Full Text] [PDF]

Response: The CD150high compartment is not the exclusive reservoir of LT-HSCs within the bone marrow
Stuart M. Chambers, David C. Weksberg, and Margaret A. Goodell
Blood 2008 111: 4414-4415. [Full Text] [PDF]



This article has been cited by other articles:


Home page
 BloodHome page
D. G. Kent, M. R. Copley, C. Benz, S. Wohrer, B. J. Dykstra, E. Ma, J. Cheyne, Y. Zhao, M. B. Bowie, Y. Zhao, et al.
Prospective isolation and molecular characterization of hematopoietic stem cells with durable self-renewal potential
Blood, June 18, 2009; 113(25): 6342 - 6350.
[Abstract] [Full Text] [PDF]

Home page
 haematolHome page
F. Cerisoli, L. Cassinelli, G. Lamorte, S. Citterio, F. Bertolotti, M. C. Magli, and S. Ottolenghi
Green fluorescent protein transgene driven by Kit regulatory sequences is expressed in hematopoietic stem cells
Haematologica, March 1, 2009; 94(3): 318 - 325.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. Tolar, A. Ishida-Yamamoto, M. Riddle, R. T. McElmurry, M. Osborn, L. Xia, T. Lund, C. Slattery, J. Uitto, A. M. Christiano, et al.
Amelioration of epidermolysis bullosa by transfer of wild-type bone marrow cells
Blood, January 29, 2009; 113(5): 1167 - 1174.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. J. Kiel, O. H. Yilmaz, and S. J. Morrison
CD150- cells are transiently reconstituting multipotent progenitors with little or no stem cell activity
Blood, April 15, 2008; 111(8): 4413 - 4414.
[Full Text] [PDF]

Home page
 BloodHome page
S. M. Chambers, D. C. Weksberg, and M. A. Goodell
Response: The CD150high compartment is not the exclusive reservoir of LT-HSCs within the bone marrow
Blood, April 15, 2008; 111(8): 4414 - 4415.
[Full Text] [PDF]


click for free articles
home about blood authors subscriptions permissions advertising public access contact us
  Copyright © 2007 by American Society of Hematology         Online ISSN: 1528-0020