|
|
Blood, 1 May 2008, Vol. 111, No. 9, pp. 4764-4770.
Prepublished online as a Blood First Edition Paper on January 3, 2008; DOI 10.1182/blood-2007-10-115915.
 Previous Article | Next Article 
Submitted October 9, 2007
Accepted December 18, 2007
Clinical quantitation of immune signature in follicular lymphoma by RT-PCR based gene expression profiling
Richard J Byers*, Ebrahim Sakhinia, Preethi Joseph, Caroline Glennie, Judith A Hoyland, Lia P Menasce, John A Radford, and Timothy Illidge
School of Cancer Imaging Sciences, Faculty of Medical and Human Sciences, University of Manchester, Manchester, United Kingdom
Molecular Diagnostic Centre, Manchester Royal Infirmary, Manchester, United Kingdom
Department of Histopathology, Manchester Royal Infirmary, Manchester, United Kingdom
Tissue Injury and Repair, School of Clinical and Laboratory Sciences, Faculty of Medical and Human Sciences, University of Manchester, Manchester, United Kingdom
Department of Histopathology, Christie Hospital NHS Foundation Trust, Manchester, United Kingdom
* Corresponding author; email: r.byers{at}manchester.ac.uk.
Microarray gene expression profiling studies have demonstrated immune response gene signatures which appear predictive of outcome in follicular lymphoma (FL). However, measurement of these marker genes in routine practice remains difficult. We have therefore investigated the immune response in FL using real-time PCR to measure expression levels of 35 candidate Indicator genes, selected from microarray studies, to polyA cDNAs prepared from 60 archived human frozen lymph nodes, in parallel with immunohistochemical analysis for CD3, CD4, CD7, CD8, CD10, CD20, CD21 & CD68. High levels of CCR1, a marker of monocyte activation, were associated with a shorter survival interval, and high levels of CD3 with better survival, whilst immunohistochemistry demonstrated association of high numbers of CD68 positive macrophages with a shorter survival interval and of high numbers of CD7 positive T-cells with a longer survival interval. The results confirm the role of the host immune response in outcome in FL and identify CCR1 as a prognostic indicator and marker of an immune switch between macrophages and a T-cell dominant response. They demonstrate the utility of polyA DNA and real-time PCR for measurement of gene signatures and the applicability of using this type of "molecular block" in clinical practice.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
Related Article in Blood Online:
-
Follicular lymphoma and the microenvironment
- Sandeep S. Dave
Blood 2008 111: 4427-4428.
[Full Text]
[PDF]
This article has been cited by other articles:
|
|
|
|
 
F. Colotta, P. Allavena, A. Sica, C. Garlanda, and A. Mantovani
Cancer-related inflammation, the seventh hallmark of cancer: links to genetic instability
Carcinogenesis,
July 1, 2009;
30(7):
1073 - 1081.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. V. Kurtova, A. T. Tamayo, R. J. Ford, and J. A. Burger
Mantle cell lymphoma cells express high levels of CXCR4, CXCR5, and VLA-4 (CD49d): importance for interactions with the stromal microenvironment and specific targeting
Blood,
May 7, 2009;
113(19):
4604 - 4613.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Maby-El Hajjami, P. Ame-Thomas, C. Pangault, O. Tribut, J. DeVos, R. Jean, N. Bescher, C. Monvoisin, J. Dulong, T. Lamy, et al.
Functional Alteration of the Lymphoma Stromal Cell Niche by the Cytokine Context: Role of Indoleamine-2,3 Dioxygenase
Cancer Res.,
April 1, 2009;
69(7):
3228 - 3237.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Burger, M. P. Quiroga, E. Hartmann, A. Burkle, W. G. Wierda, M. J. Keating, and A. Rosenwald
High-level expression of the T-cell chemokines CCL3 and CCL4 by chronic lymphocytic leukemia B cells in nurselike cell cocultures and after BCR stimulation
Blood,
March 26, 2009;
113(13):
3050 - 3058.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Lejeune and T. Alvaro
Clinicobiological, prognostic and therapeutic implications of the tumor microenvironment in follicular lymphoma
Haematologica,
January 1, 2009;
94(1):
16 - 21.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Mantovani
Cancer-related Inflammation: The Seventh Hallmark of Cancer
ASCO Educational Book,
January 1, 2009;
2009(1):
723 - 726.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. O'Shea, C. O'Riain, C. Taylor, R. Waters, E. Carlotti, F. MacDougall, J. Gribben, A. Rosenwald, G. Ott, L. M. Rimsza, et al.
The presence of TP53 mutation at diagnosis of follicular lymphoma identifies a high-risk group of patients with shortened time to disease progression and poorer overall survival
Blood,
October 15, 2008;
112(8):
3126 - 3129.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. A. Johnson and R. D. Gascoyne
Gene expression signatures in follicular lymphoma: are they ready for the clinic?
Haematologica,
July 1, 2008;
93(7):
982 - 987.
[Full Text]
[PDF]
|
|
|
|
|
