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Blood, 15 June 2008, Vol. 111, No. 12, pp. 5544-5552. Prepublished online as a Blood First Edition Paper on February 11, 2008; DOI 10.1182/blood-2007-10-119073. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2007-10-119073v1 111/12/5544    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Toth, Z. E. Articles by Mezey, E. Search for Related Content PubMed PubMed Citation Articles by Toth, Z. E. Articles by Mezey, E. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted October 18, 2007 Accepted February 5, 2008 The combination of granulocyte colony stimulatory factor and stem cell factor significantly increases the number of bone marrow derived endothelial cells in brains of mice following cerebral ischemia Zsuzsanna E. Toth, Ronen Leker, Tal Shahar, Sandra Pastorino, Ildiko Szalayova, Brook Asemenew, Sharon Key, Alissa Parmelee, Balazs Mayer, Krisztian Nemeth, Andras Bratincsak, and Eva Mezey* NIDCR, NIH, Bethesda, MD NINDS, NIH, Bethesda, MD NCI, NIH, Bethesda, MD NIMH, NIH, Bethesda, MD * Corresponding author; email: mezeye{at}mail.nih.gov. Granulocyte colony stimulating factor (GCSF) induces proliferation of bone marrow derived cells. GCSF is neuroprotective after experimental brain injury but the mechanisms involved remain unclear. Stem Cell Factor (SCF) is a cytokine important for the survival and differentiation of hematopoietic stem cells. Its receptor (c-kit or CD117) is present in some endothelial cells. We aimed to determine whether the combination of GCSF/SCF induces angiogenesis in the CNS by promoting entry of endothelial precursors into the injured brain and causing them to proliferate there. We induced permanent middle cerebral artery occlusion in female mice that previously underwent gender-mismatched bone marrow transplantation from EGFP expressing mice. GCSF/SCF treatment reduced infarct volumes by more than 50% and resulted in a 1.5 fold increase in vessel formation in mice with stroke; a large percentage of which contain endothelial cells of bone marrow origin. Most cells entering the brain maintained their bone marrow identity and did not transdifferentiate into neural cells. GCSF/SCF treatment also led to a 2-fold increase in the number of newborn cells in the ischemic hemisphere. These finding suggests that GCSF/SCF treatment might help recovery through induction of bone marrow derived angiogenesis thus improving neuronal survival and functional outcome. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Blood to brain yet again Dov Zipori Blood 2008 111: 5422-5423. [Full Text] [PDF] This article has been cited by other articles: B. T. Bratane, J. Bouley, A. Schneider, B. Bastan, N. Henninger, and M. Fisher Granulocyte-Colony Stimulating Factor Delays PWI/DWI Mismatch Evolution and Reduces Final Infarct Volume in Permanent-Suture and Embolic Focal Cerebral Ischemia Models in the Rat Stroke, September 1, 2009; 40(9): 3102 - 3106. [Abstract] [Full Text] [PDF]

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