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Blood, 1 March 2008, Vol. 111, No. 5, pp. 2636-2639. Prepublished online as a Blood First Edition Paper on January 3, 2008; DOI 10.1182/blood-2007-11-124685. This Article Full Text (PDF) All Versions of this Article: blood-2007-11-124685v1 111/5/2636    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Tober, J. Articles by Palis, J. Search for Related Content PubMed PubMed Citation Articles by Tober, J. Articles by Palis, J. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted November 19, 2007 Accepted December 24, 2007 Primitive erythropoiesis and megakaryopoiesis in the yolk sac are independent of c-myb Joanna Tober, Kathleen E. McGrath, and James Palis* Center for Pediatric Biomedical Research, University of Rochester Medical Center, Rochester, NY, United States Department of Pediatrics, University of Rochester Medical Center, Rochester, NY, United States * Corresponding author; email: james_palis{at}urmc.rochester.edu. Hematopoiesis initiates within the yolk sac of mammalian embryos in overlapping primitive and definitive waves each containing erythroid and megakaryocyte lineages. c-myb-null mouse fetuses lack definitive erythrocytes but contain circulating primitive erythroblasts and hepatic megakaryocytes. However, it is unclear if c-myb-null embryos harbor definitive erythroid or megakaryocyte progenitors. We determined that c-myb is not expressed in primitive erythroid precursors and that c-myb-null embryos have normal primitive erythroid and megakaryocyte progenitor numbers and kinetics between E7.0-E9.0. While primitive hematopoiesis is c-myb-independent, no definitive erythroid potential was detected in c-myb-null embryos, confirming that definitive erythropoiesis, beginning at E8.25 in the yolk sac, is completely c-myb-dependent. In contrast, reduced numbers of megakaryocyte progenitors with restricted proliferative capacity persist in E10.5 yolk sac and E11.5 liver. Despite this impaired megakaryocyte potential, c-myb-null fetuses have normal platelet numbers at E12.5 but became thrombocytopenic by E15.5, suggesting that c-myb is required for sustained thrombopoiesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: O. Klimchenko, M. Mori, A. DiStefano, T. Langlois, F. Larbret, Y. Lecluse, O. Feraud, W. Vainchenker, F. Norol, and N. Debili A common bipotent progenitor generates the erythroid and megakaryocyte lineages in embryonic stem cell-derived primitive hematopoiesis Blood, August 20, 2009; 114(8): 1506 - 1517. [Abstract] [Full Text] [PDF]

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