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 Blood, 15 August 2008, Vol. 112, No. 4, pp. 1048-1055.
Prepublished online as a Blood First Edition Paper on May 21, 2008; DOI 10.1182/blood-2007-11-125385.

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Submitted November 21, 2007
Accepted April 6, 2008

B-lineage commitment prior to surface expression of B220 and CD19 on hematopoietic progenitor cells

Robert Mansson, Sasan Zandi, Kristina Andersson, Inga-Lill Martensson, Sten Eirik W. Jacobsen, David Bryder, and Mikael Sigvardsson*

Biomedicine and Surgery, Linkoping University, Linköping, Sweden
Hematopoietic Stem Cell Laboratory, Lund University, Lund, Sweden
Lymphocyte Signaling and Development, Barbraham Institute, Cambridge, United Kingdom
Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, Lund, Sweden
Immunology, Lund University, Lund, Sweden

* Corresponding author; email: mikael.sigvardsson{at}ibk.liu.se.

Commitment of hematopoietic progenitor cells to B-lymphoid cell fate has been suggested to coincide with the development of PAX5 expressing B220+CD19+ pro-B cells. We have used a transgenic reporter mouse, expressing human CD25 under the control of the B-lineage restricted Igll1 ({lambda}5) promoter to investigate the lineage potential of early progenitor cells in the bone marrow. This strategy allowed us to identify a reporter expressing LIN-B220-CD19-CD127+FLT3+SCA1lowKITlow population that display a lack of myeloid and a 90% reduction in in vitro T-cell potential as compared to their reporter negative counterpart. Gene expression analysis demonstrated that these lineage restricted cells express B-lineage associated genes to levels comparable to that observed in pro-B cells. These data suggest that B-lineage commitment occur before the expression of B220 and CD19.


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