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Blood, 15 July 2008, Vol. 112, No. 2, pp. 350-361. Prepublished online as a Blood First Edition Paper on May 6, 2008; DOI 10.1182/blood-2007-12-128215. This Article Full Text (PDF) Supplemental Methods and Figures All Versions of this Article: blood-2007-12-128215v1 112/2/350    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zeoli, A. Articles by Brizzi, M. F. Search for Related Content PubMed PubMed Citation Articles by Zeoli, A. Articles by Brizzi, M. F. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted December 12, 2007 Accepted March 24, 2008 Interleukin-3 (IL-3) promotes expansion of hemopoietic-derived CD45+ angiogenic cells and their arterial commitment via STAT5 activation Annarita Zeoli, Patrizia Dentelli, Arturo Rosso, Gabriele Togliatto, Antonella Trombetta, Laura Damiano, Paola Francia di Celle, Luigi Pegoraro, Fiorella Altruda, and Maria Felice Brizzi* Department of Internal Medicine, University of Torino, Torino Department of Genetics, Biology and Biochemistry, Molecular Biotechnology Center, University of Torino, Torino Center for Experimental Research and Clinical Studies (CERMS), Azienda Ospedaliera S. Giovanni Battista, Torino * Corresponding author; email: mariafelice.brizzi{at}unito.it. Interleukin-3 (IL-3) released by infiltrating inflammatory cells in different pathological settings contributes to organ and tumor angiogenesis. Herein we demonstrate that IL-3 expands a subset of CD45+ circulating angiogenic cells clonally derived from the hemopoietic progenitors. Moreover, CD45+ cells exposed to IL-3 acquire arterial specification and directly contribute to the formation of vessels in vivo. Depletion of STAT5 provides evidences that IL-3-mediated cell expansion and arterial morphogenesis rely on STAT5 activation. In addition, by means of Tie2-transgenic mice we demonstrate that STAT5 also regulates IL-3-induced expansion and arterial specification of bone-marrow-derived CD45+ cells. Thus, our data provide the first evidence that, in inflammatory microenvironment containing IL-3, angiogenic cells derived from hemopoietic precursors can act as adult vasculogenic cells. Moreover, the characterization of the signalling pathway regulating these events provides the rational for therapeutically targeting STAT5 in these pathological settings. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Zampetaki and Q. Xu Vascular Remodeling in Diabetes: Don't Leave Without Your STAT5 Arterioscler Thromb Vasc Biol, January 1, 2009; 29(1): 10 - 11. [Full Text] [PDF] P. Dentelli, A. Trombetta, G. Togliatto, A. Zeoli, A. Rosso, B. Uberti, F. Orso, D. Taverna, L. Pegoraro, and M. F. Brizzi Formation of STAT5/PPAR{gamma} Transcriptional Complex Modulates Angiogenic Cell Bioavailability in Diabetes Arterioscler Thromb Vasc Biol, January 1, 2009; 29(1): 114 - 120. [Abstract] [Full Text] [PDF]

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