|
|
Blood, 1 July 2008, Vol. 112, No. 1, pp. 73-81.
Prepublished online as a Blood First Edition Paper on April 29, 2008; DOI 10.1182/blood-2007-12-128835.
 Previous Article | Next Article 
Submitted December 13, 2007
Accepted April 2, 2008
Role of ephrinB2 expression in endothelial cells during arteriogenesis: Impact on smooth muscle cell migration and monocyte recruitment
Thomas Korff, Jennifer Braun, Dennis Pfaff, Hellmut G Augustin, and Markus Hecker*
Institute of Physiology and Pathophysiology, University Hospital Heidelberg, Heidelberg, Germany
Joint Research Division Vascular Biology, German Cancer Research Center, Heidelberg, Germany
* Corresponding author; email: hecker{at}physiologie.uni-hd.de.
Expression of the arterial marker gene ephrinB2 in endothelial cells is a prerequisite for adequate remodeling processes of the developing or angiogenic vasculature. Although its role in these processes has been extensively studied, the impact of ephrinB2 on the remodeling of adult arteries is largely unknown. To this end, we analyzed its expression during a biomechanically induced arteriolar remodeling process known as arteriogenesis and noted a significant increase in ephrinB2 expression under these conditions. By examining those biomechanical forces presumed to drive arteriogenesis, we identified cyclic stretch as a critical inducer of ephrinB2 expression in endothelial cells. Subsequent functional analyses in vitro revealed that endothelial cells expressing ephrinB2 limit the migration of smooth muscle cells, thereby enhancing segregation of both cell types. Moreover, MCP-1 induced transmigration of monocytes through a monolayer of endothelial cells over-expressing a truncated variant of ephrinB2 was clearly impeded. Taken together, these data suggest that expression of ephrinB2 in adult endothelial cells is up-regulated during arterial remodeling and controlled by cyclic stretch - a well known inducer of such processes. This stretch-induced ephrinB2 expression may be pivotal for arteriogenesis as it limits smooth muscle cell migration within defined borders and controls monocyte extravasation.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
O. Salvucci, D. Maric, M. Economopoulou, S. Sakakibara, S. Merlin, A. Follenzi, and G. Tosato
EphrinB reverse signaling contributes to endothelial and mural cell assembly into vascular structures
Blood,
August 20, 2009;
114(8):
1707 - 1716.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Pfaff, M. Heroult, M. Riedel, Y. Reiss, R. Kirmse, T. Ludwig, T. Korff, M. Hecker, and H. G. Augustin
Involvement of endothelial ephrin-B2 in adhesion and transmigration of EphB-receptor-expressing monocytes
J. Cell Sci.,
November 15, 2008;
121(22):
3842 - 3850.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
