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Blood, 15 May 2008, Vol. 111, No. 10, pp. 5078-5085. Prepublished online as a Blood First Edition Paper on March 12, 2008; DOI 10.1182/blood-2008-01-133355. This Article Full Text (PDF) Supplemental Tables and Figures All Versions of this Article: blood-2008-01-133355v1 111/10/5078    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jongen-Lavrencic, M. Articles by Lowenberg, B. Search for Related Content PubMed PubMed Citation Articles by Jongen-Lavrencic, M. Articles by Lowenberg, B. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 11, 2008 Accepted March 5, 2008 MicroRNA expression profiling in relation to the genetic heterogeneity of acute myeloid leukemia Mojca Jongen-Lavrencic*, Su Ming Sun, Menno K Dijkstra, Peter JM Valk, and Bob Lowenberg Department of Hematology, Erasmus University Medical Center, Rotterdam, Netherlands * Corresponding author; email: m.lavrencic{at}erasmusmc.nl. Acute myeloid leukemia (AML) is a highly diverse disease characterized by various cytogenetic and molecular abnormalities. MicroRNAs are small non-coding RNAs that show variable expression during myeloid differentiation. MicroRNAs expression in marrow blasts in 215 cases of newly diagnosed and (cyto)genetically defined AML was assessed using quantitative RT-PCR for 260 known human microRNAs. In the same series mRNA gene expression profiles were established, allowing a direct comparison between microRNA and mRNA expression. We show that microRNA expression profiling following unsupervised analysis reveals distinctive microRNA signatures that correlate with cytogenetic and molecular subtypes of AML i.e AMLs with t(8;21), t(15;17), inv(16), NPM1 and CEBPA mutations. Significantly differentially expressed microRNAs for genetic subtypes of AML were identified. Specific microRNAs with established oncogenic and tumor suppressor functions, such as microRNA-155, microRNA-21, let-7 appear to be associated with particular subtypes. Combinations of selected sets of microRNAs could predict cytogenetically normal AML with mutations in the genes of NPM1 and CEBPA and FLT3-ITD with similar accuracy as mRNA probe set combinations defined by gene expression profiling (GEP). MicroRNA expression apparently bears specific relationships to the heterogeneous pathobiology of AML. Distinctive microRNA signatures appear of potential value in the clinical diagnosis of AML. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Haferlach, C. Mecucci, S. Schnittger, A. Kohlmann, M. Mancini, A. Cuneo, N. Testoni, G. Rege-Cambrin, A. Santucci, M. Vignetti, et al. AML with mutated NPM1 carrying a normal or aberrant karyotype show overlapping biologic, pathologic, immunophenotypic, and prognostic features Blood, October 1, 2009; 114(14): 3024 - 3032. [Abstract] [Full Text] [PDF] A. A. van de Loosdrecht, C. Alhan, M. C. Bene, M. G. Della Porta, A. M. Drager, J. Feuillard, P. Font, U. Germing, D. Haase, C. H. Homburg, et al. Standardization of flow cytometry in myelodysplastic syndromes: report from the first European LeukemiaNet working conference on flow cytometry in myelodysplastic syndromes Haematologica, August 1, 2009; 94(8): 1124 - 1134. [Abstract] [Full Text] [PDF] M. Pigazzi, E. Manara, E. Baron, and G. Basso miR-34b Targets Cyclic AMP-Responsive Element Binding Protein in Acute Myeloid Leukemia Cancer Res., March 15, 2009; 69(6): 2471 - 2478. [Abstract] [Full Text] [PDF] B. J. Wouters, B. Lowenberg, and R. Delwel A decade of genome-wide gene expression profiling in acute myeloid leukemia: flashback and prospects Blood, January 8, 2009; 113(2): 291 - 298. [Abstract] [Full Text] [PDF] F. Kuchenbauer, R. D. Morin, B. Argiropoulos, O. I. Petriv, M. Griffith, M. Heuser, E. Yung, J. Piper, A. Delaney, A.-L. Prabhu, et al. In-depth characterization of the microRNA transcriptome in a leukemia progression model Genome Res., November 1, 2008; 18(11): 1787 - 1797. [Abstract] [Full Text] [PDF] Z. Li, J. Lu, M. Sun, S. Mi, H. Zhang, R. T. Luo, P. Chen, Y. Wang, M. Yan, Z. Qian, et al. Distinct microRNA expression profiles in acute myeloid leukemia with common translocations PNAS, October 7, 2008; 105(40): 15535 - 15540. [Abstract] [Full Text] [PDF] B. Lowenberg Acute Myeloid Leukemia: The Challenge of Capturing Disease Variety Hematology, January 1, 2008; 2008(1): 1 - 11. [Abstract] [Full Text] [PDF]

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