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Blood, 15 June 2008, Vol. 111, No. 12, pp. 5621-5628.
Prepublished online as a Blood First Edition Paper on April 1, 2008; DOI 10.1182/blood-2008-01-134155.
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Submitted January 15, 2008
Accepted March 21, 2008
Capture of plasma membrane fragments from target cells by trogocytosis requires signaling in T cells but not in B cells
Anne Aucher, Eddy Magdeleine, Etienne Joly, and Denis Hudrisier*
Institut de Pharmacologie et Viologie Structurale, Centre National de Recherche Scientifique, Toulouse, France
Universite Paul Sabatier, IPBS, CNRS UMR5089, Toulouse, France
* Corresponding author; email: denis.hudrisier{at}ipbs.fr.
Upon recognition of their respective cellular partners, T and B cells acquire their antigens by a process of membrane capture called trogocytosis. Here, we report that various inhibitors of actin polymerization or of kinases involved in intracellular signaling, partially or fully inhibited trogocytosis by CD8+ and CD4+ T cells, whereas they had no effect on trogocytosis by B cells. Similarly, trogocytosis by T cells was inhibited at 4°C, whereas in B cells it was independent of temperature, indicating that trogocytosis by B cells does not rely on active processes. By contrast, most inhibitors we tested impaired both T cell and B cell activation. The differential effect of inhibitors on T cell and B cell trogocytosis was not due to the higher affinity of the B cell receptor for its cognate antigen when compared to the affinity of the T cell receptor for its own antigen, but it correlated tightly with the abilities of T cells and B cells to form conjugates with their target cells in the presence of inhibitors. Trogocytosis thus has different requirements in different cell types. Moreover, the capture of membrane antigen by B cells is identified as a novel signaling-independent event of B cell biology.
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