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Blood, 15 August 2008, Vol. 112, No. 4, pp. 1280-1289. Prepublished online as a Blood First Edition Paper on June 4, 2008; DOI 10.1182/blood-2008-01-134429. This Article Full Text (PDF) Supplemental Videos All Versions of this Article: blood-2008-01-134429v1 112/4/1280    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Stefanidakis, M. Articles by Luscinskas, F. W Search for Related Content PubMed PubMed Citation Articles by Stefanidakis, M. Articles by Luscinskas, F. W Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 18, 2008 Accepted May 17, 2008 Endothelial CD47 interaction with SIRP is required for human T cell transendothelial migration under shear flow conditions in vitro Michael Stefanidakis, Gail Newton, Winston Y Lee, Charles A Parkos, and Francis W Luscinskas* Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, United States Pathology, Emory University, Georgia, GA, United States * Corresponding author; email: fluscinskas{at}rics.bwh.harvard.edu. Leukocyte transendothelial migration (TEM) is a critical event during inflammation. CD47 has been implicated in myeloid cell migration across endothelium and epithelium. CD47 binds to signal regulatory protein (SIRP) SIRP and SIRP. So far, little is known about the role of endothelial CD47 in T cell TEM in vivo or under flow conditions in vitro. FACS and biochemical analysis show that CD3+ T cells express SIRP but not SIRP and fluorescence microscopy revealed that CD47 was enriched at endothelial junctions. These expression patterns suggested that CD47 plays a role in T cell TEM through binding interactions with SIRP. We tested, therefore, whether CD47-SIRP interactions affect T cell transmigration using blocking mAb against CD47 or SIRP in an in vitro flow model. These antibodies inhibited T cell TEM by 70 ± 6% and 82 ± 1%, respectively, but had no effect on adhesion. In agreement with human mAb studies, transmigration of murine wild-type Th1 cells across TNF- activated murine CD47-/- endothelium was reduced by 75 ± 2% even though murine T cells appear to lack SIRP. None-the-less, these findings suggest endothelial cell CD47 interacting with T cell ligands, such as SIRP, play an important role in T cell transendothelial migration. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: W. A. Muller Mechanisms of Transendothelial Migration of Leukocytes Circ. Res., July 31, 2009; 105(3): 223 - 230. [Abstract] [Full Text] [PDF]

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